Abstract
Leptospirosis causes significant economic loss within the cattle industry worldwide. Current diagnostic methods are generally inadequate for dealing with large numbers of samples, are outdated, and provide little useful diagnostic and epidemiological information. This aim of this study was to apply a microsphere immunoassay (MIA), utilising Luminex xMap technology, to 200 bovine serum samples to determine this method's usefulness in leptospirosis diagnosis in comparison with the current gold standard, the microscopic agglutination test (MAT). Although MAT is the most widely used laboratory test for the diagnosis of leptospirosis, its reliance on live cultures, subjective interpretation of results and an inability to differentiate between antibody classes, suggest MAT is no longer the best method for the diagnosis of leptospirosis. The results presented in this paper show that MIA was able to determine reactive from non-reactive samples when compared with MAT, and was able to differentiate IgG and IgM classes of antibody. The results suggest increased sensitivity in MIA and the ability to multiplex up to 500 antigens at one time allows for significant improvements in cost-effectiveness as well as a reduced dependency on live cul...Continue Reading
References
Jun 1, 1979·New Zealand Veterinary Journal·R B MarshallJ S Hellstrom
Jan 1, 1990·Veterinary Microbiology·Z BercovichB A Bokhout
Jul 26, 1986·The Veterinary Record·S C HathawayD G Pritchard
Aug 1, 1985·Journal of Dairy Science·B R BellO W Robison
Aug 1, 1985·Veterinary Microbiology·D V CousinsL Hustas
Oct 1, 1982·Pathology·V TuS Faine
Feb 13, 1982·The Veterinary Record·W A EllisJ Hanna
Mar 1, 1982·Australian Veterinary Journal·J D AllenC R Wilks
Apr 1, 1981·Journal of Wildlife Diseases·A R MilnerP J Presidente
Jun 27, 1981·The Veterinary Record·W A EllisJ Cassells
Jul 1, 1980·Australian Veterinary Journal·A R MilnerK Calvert
Nov 1, 1994·The Veterinary Clinics of North America. Food Animal Practice·W A Ellis
Sep 1, 1994·Australian Veterinary Journal·C R SmithB G Corney
Oct 1, 1993·Australian Veterinary Journal·C S McClintockM Woodrow
Mar 1, 1996·Revue Scientifique Et Technique·I A GardnerW M Boyce
Nov 14, 1998·Journal of Wildlife Diseases·R J MasonM L Symonds
Jul 17, 2001·American Journal of Veterinary Research·C A Bolin, D P Alt
Jul 1, 1982·New Zealand Veterinary Journal·R W Worthington
Oct 10, 2008·International Journal of Systematic and Evolutionary Microbiology·B G CorneyL D Smythe
Aug 7, 2010·Journal of Wildlife Diseases·Michael W RobertsAndrew Slack
Mar 26, 2015·PLoS Neglected Tropical Diseases·Sarah J WynwoodScott B Craig
Apr 22, 2016·Journal of AOAC International
Citations
Feb 9, 2019·Science·Xu A ZhangYuHuang Wang
Oct 19, 2019·Journal of Microbiological Methods·Israel Barbosa GuedesMarcos Bryan Heinemann
Nov 17, 2021·PLoS Neglected Tropical Diseases·Shabani Kiyabo MottoElizabeth Anne Jessie Cook