Serological differentiation between COVID-19 and SARS infections.

Emerging Microbes & Infections
Wan Ni ChiaLin-Fa Wang

Abstract

In response to the coronavirus disease 2019 (COVID-19) outbreak, caused by SARS-CoV-2, multiple diagnostic tests are required for acute disease diagnosis, contact tracing, monitoring asymptomatic infection rates and assessing herd immunity. While PCR remains the frontline test of choice in the acute diagnostic setting, serological tests are urgently needed. Unlike PCR tests which are highly specific, cross-reactivity is a major challenge for COVID-19 antibody tests considering there are six other coronaviruses known to infect humans. SARS-CoV is genetically related to SARS-CoV-2 sharing approximately 80% sequence identity and both belong to the species SARS related coronavirus in the genus Betacoronavirus of family Coronaviridae. We developed and compared the performance of four different serological tests to comprehensively assess the cross-reactivity between COVID-19 and SARS patient sera. There is significant cross-reactivity when N protein of either virus is used. The S1 or RBD regions from the spike (S) protein offers better specificity. Amongst the different platforms, capture ELISA performed best. We found that SARS survivors all have significant levels of antibodies remaining in their blood 17 years after infection. Ant...Continue Reading

References

Sep 15, 2000·Journal of Immunological Methods·D A Vignali
Oct 3, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·J S M Peiris
Jan 25, 2005·Emerging Infectious Diseases·Changchun TuLin-Fa Wang
Oct 1, 2005·Science·Wendong LiLin-Fa Wang
Jun 8, 2006·Journal of Clinical Microbiology·Susanna K P LauKwok-yung Yuen
Aug 17, 2006·FEMS Microbiology Reviews·Lia van der HoekBen Berkhout
Jun 23, 2009·Immunology Letters·Rachael Racine, Gary M Winslow
Jun 4, 2010·Expert Review of Vaccines·Peter D BurbeloMichael J Iadarola
Oct 19, 2012·The New England Journal of Medicine·Ali M ZakiRon A M Fouchier
Aug 13, 2013·The Lancet Infectious Diseases·Chantal B E M ReuskenMarion P G Koopmans
Mar 25, 2014·Emerging Infectious Diseases·Benjamin MeyerChristian Drosten
Jan 28, 2020·Lancet·Chen WangGeorge F Gao
Mar 4, 2020·Nature Microbiology·UNKNOWN Coronaviridae Study Group of the International Committee on Taxonomy of Viruses
Mar 4, 2020·JAMA : the Journal of the American Medical Association·Barnaby Edward YoungUNKNOWN Singapore 2019 Novel Coronavirus Outbreak Research Team
Apr 1, 2020·Nature·Jian ShangFang Li
Apr 5, 2020·Journal of Clinical Microbiology·Yi-Wei TangCharles W Stratton
Apr 6, 2020·Lancet·Anna Petherick
Apr 7, 2020·Trends in Immunology·Shibo JiangLanying Du
Apr 9, 2020·Emerging Infectious Diseases·Nisreen M A OkbaBart L Haagmans
May 14, 2020·Nature Medicine·Fatima AmanatFlorian Krammer

❮ Previous
Next ❯

Citations

Aug 14, 2020·Journal of Medical Virology·I-Ching SamYoke Fun Chan
Nov 11, 2020·Biochemical Society Transactions·Jiayue-Clara Jiang, Yan Zhang
Nov 26, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·María Simón SacristanMaría Mateo Maestre
Nov 17, 2020·Transboundary and Emerging Diseases·Kerstin WernikeMartin Beer
Dec 30, 2020·Klinicheskaia laboratornaia diagnostika·Boris Georgievich Andryukov, I N Lyapun
Nov 1, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Hadi M YassineGheyath K Nasrallah
Feb 14, 2021·Communications Biology·Nischay MishraW Ian Lipkin
Nov 5, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Lyle R PetersenLara J Akinbami
Mar 6, 2021·Emerging Microbes & Infections·Kelvin Kai-Wang ToKwok-Yung Yuen
Jan 10, 2021·Journal of Clinical Microbiology·Nathalie RenardFranck Berthier
Mar 10, 2021·Transboundary and Emerging Diseases·Sergio Villanueva-SazAntonio Fernández
Mar 23, 2021·Journal of Virological Methods·Reza SoleimaniBenoît Kabamba-Mukadi
Mar 19, 2021·Annals of the Academy of Medicine, Singapore·Glorijoy Shi En TanShawn Vasoo
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Jacobo GinerAntonio Fernández
Apr 20, 2021·Frontiers in Plant Science·Mohau S MakatsaWendy A Burgers
May 5, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hidetsugu FujigakiKuniaki Saito
May 15, 2021·Frontiers in Medicine·Naheed ChoudhryPatrick T Kennedy
Jun 29, 2021·Frontiers in Immunology·Siggeir F BrynjolfssonBjorn R Ludviksson
Aug 28, 2021·Journal of Pharmacy & Bioallied Sciences·Arjun B RaviR Kavitha
Jul 5, 2021·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Louis GrandjeanUNKNOWN Co-Stars Study Team
Sep 29, 2021·European Journal of Immunology·Anu HaveriMerit Melin
Nov 7, 2021·Journal of Clinical Laboratory Analysis·Lin ShenZhencheng Chen
Dec 3, 2021·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Jorge PulidoLuis A Rivas
Dec 18, 2021·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Aránzazu Caballero-MarcosMagdalena Salcedo

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ELISA
immunoprecipitation
ELISAs

Software Mentioned

PhyML
Clustal Omega
GraphPad Prism
Geneious Prime
Luminex
GenScript

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
A BerminghamM C Zambon
Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
David S Y OngEuropean Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group for Respiratory Viruses (ESGREV)
© 2022 Meta ULC. All rights reserved