Serological Evidence of Mosquito-Borne Flaviviruses Circulation in Hunting Dogs in Campania Region, Italy

Vector Borne and Zoonotic Diseases
Serena MontagnaroUgo Pagnini

Abstract

A Flavivirus survey on 183 hunting dogs was conducted in Campania region, Southern Italy. The seroprevalence value of 40.43% (74/183, 95% confidence intervals [CIs] 33.37-47.49) detected in our study using a competitive enzyme-linked immunosorbent serologic assay (cELISA) proves a considerable level of Flavivirus exposition of these animals. Among the 74 cELISA-positive sera, seroneutralization (SN) test showed that 24 sera resulted positive for Usutu virus with an overall prevalence of 13.11% (24/183) (95% CI 8.27-17.95), but none of cELISA-positive samples resulted positive for West Nile virus. Data analysis showed a significant difference of cELISA seropositivity risk factors in case of presence of farm animals in contact with hunting dogs and for dogs living in a rural environment but not for gender, age, management, hunting season, and hunting abroad. A RT-PCR assay was performed to detect the Flavivirus RNA, but none of the blood samples tested positive. This study documents the first report regarding the circulation of Flavivirus in hunting dog in Southern Italy and suggests the dog as an interesting target to monitor Flavivirus circulation.

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Citations

Mar 20, 2020·Animals : an Open Access Journal From MDPI·Angela PetruccelliSerena Montagnaro
Jul 25, 2019·Viruses·Ferdinand RoeschMarco Vignuzzi
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Aug 30, 2020·Pathogens·Tatjana Vilibic-CavlekGiovanni Savini
Dec 4, 2020·Pathogens·Orianne ConstantYannick Simonin
Jun 2, 2021·The Veterinary Clinics of North America. Small Animal Practice·Marcella Ridgway

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Methods Mentioned

BETA
enzyme-linked immunosorbent serologic assay
electrophoresis
Assay

Software Mentioned

MedCalc

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