Serological identification of endothelial antigens predominantly recognized in Kawasaki disease patients by recombinant expression cloning

Microbiology and Immunology
Miho KanekoEiichi Nakayama

Abstract

We showed IgG immune response against endothelial antigens in sera obtained from convalescent Kawasaki disease (KD) patients after recovery from the disease during the follow-up period using serological analysis of recombinant cDNA expression library (SEREX) methodology. We identified 46 antigens represented by 69 clones by immunoscreening of a cDNA expression library from tumor necrosis factor-alpha (TNFalpha) treated human umbilical vein endothelial cells (HUVEC) with sera from 4 KD patients. They included ubiquitin pathway proteins, transcriptional factors, signal transduction molecules, metabolic enzymes, cytoskeletal proteins, an adhesion molecule, and a cell cycle protein. By serological survey using phage plaque assay, sera from 5 non-KD patients were rarely reactive with the antigens. Among the antigens, tropomyosin was most frequently isolated (18 of 69 clones). Seventeen of the 18 clones were identified using KD3 serum. The second most frequently isolated antigen was also a cytoskeletal protein, called Tplastin (3 of 69 clones).

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J AbeD Y Leung
Jun 1, 1992·Archives of Disease in Childhood·S FurukawaK Yabuta
Dec 1, 1991·Acta Paediatrica Japonica; Overseas Edition·H KikutaT Osato
Aug 1, 1990·Clinical Immunology and Immunopathology·S FurukawaK Yabuta
Jul 30, 1993·International Journal of Cancer. Journal International Du Cancer·K IwaiT Yoshiki
Dec 1, 1994·The Journal of Cell Biology·M ArpinD Louvard
Mar 1, 1993·The Journal of Experimental Medicine·J AbeD Y Leung
Apr 1, 1994·Molecular and Cellular Biology·C S LinP Matsudaira
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·U SahinM Pfreundschuh
Jan 27, 1998·The Journal of Biological Chemistry·F Ramos-MoralesR M Rios
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·D ZhouJ E Galán
Nov 28, 2000·International Journal of Cancer. Journal International Du Cancer·T OnoE Nakayama
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A H RowleyS C Baker
May 2, 2002·Pediatrics International : Official Journal of the Japan Pediatric Society·Seiichiro TakeshitaIsao Sekine
Jun 27, 2002·Biochemical and Biophysical Research Communications·Yoon LimDoo-Il Jeoung
Oct 23, 2002·Clinical and Experimental Immunology·E GrunebaumY Shoenfeld
Oct 11, 2003·Autoimmunity Reviews·Philippe KrebsBurkhard Ludewig

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Citations

Jan 30, 2008·Clinical Reviews in Allergy & Immunology·Philippe Guilpain, Luc Mouthon
Dec 8, 2010·Expert Opinion on Biological Therapy·Rie KarasawaTomohiro Kato
Mar 6, 2010·Pediatrics International : Official Journal of the Japan Pediatric Society·Sakda Arj-OngJohn Attia
Apr 15, 2014·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Alexis RégentLuc Mouthon
Nov 1, 2007·Expert Review of Clinical Immunology·Ju Hee LeeKwang Hoon Lee
Jun 1, 2018·Neurologia Medico-chirurgica·Yoichi YoshidaYasuo Iwadate
Sep 8, 2010·Nihon Rinshō Men'eki Gakkai kaishi = Japanese journal of clinical immunology·Rie KarasawaKazuo Yudoh
Nov 7, 2021·Scientific Reports·Masaaki KubotaYasuo Iwadate

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