Serological survey of Seewis virus antibodies in patients suspected for hantavirus infection in Finland; a cross-reaction between Puumala virus antiserum with Seewis virus N protein?

The Journal of General Virology
Jiaxin LingJussi Hepojoki

Abstract

Puumala virus (PUUV, carried by Myodes glareolus) co-circulates with Seewis virus (SWSV, carried by Sorex araneus) in Finland. While PUUV causes 1000-3000 nephropathia epidemica (NE) cases annually, the pathogenicity of SWSV to man is unknown. To study the prevalence of SWSV antibodies in hantavirus fever-like patients' sera, we used recombinant SWSV nucleocapsid (N) protein as the antigen in ELISA, immunofluorescence assay (IFA) and immunoblotting. While characterizing the recombinant SWSV N protein, we observed that a polyclonal rabbit antiserum against PUUV N protein cross-reacted with SWSV N protein and vice versa. We initially screened 486 (450 PUUV-seronegative and 36 PUUV-seropositive) samples sent to Helsinki University Hospital Laboratory for PUUV serodiagnosis during 2002 and 2007 in an SWSV N protein IgG ELISA. In total, 4.2 % (19/450) of the PUUV-seronegative samples were reactive in the SWSV N protein IgG ELISA and none of the tested samples [43 PUUV-seronegative (weakly reactive in the SWSV IgG ELISA) and 15 random] were reactive in the SWSV N protein IgM ELISA. None of the IgG reactions could be confirmed by IFA or immunoblotting. Furthermore, among the 36 PUUV-seropositive samples three were reactive in SWSV N p...Continue Reading

References

Jun 17, 1995·Lancet·O VapalahtiH Henttonen
Mar 22, 2002·The Journal of General Virology·Xiao-Dong LiHilkka Lankinen
Sep 27, 2003·Journal of Virology·Pasi KaukinenAlexander Plyusnin
Oct 31, 2007·Virology Journal·Jin-Won SongRichard Yanagihara
Jan 25, 2008·Emerging Infectious Diseases·Jin-Won SongRichard Yanagihara
May 9, 2008·Reviews in Medical Virology·Antti VaheriAlexander Plyusnin
Apr 9, 2010·Clinical Microbiology Reviews·Colleen B JonssonOlli Vapalahti
Apr 30, 2010·Vector Borne and Zoonotic Diseases·Liudmila N YashinaRichard Yanagihara
May 25, 2012·The Journal of General Virology·Jussi HepojokiAntti Vaheri
Jul 26, 2012·Archives of Virology·Mathias SchlegelBernd Köllner
Feb 15, 2013·PLoS Pathogens·Wen-Ping GuoYong-Zhen Zhang
Jul 31, 2013·Virus Research·Katarina ResmanTatjana Avšič Zupanc
Sep 11, 2013·Nature Reviews. Microbiology·Antti VaheriJukka Mustonen
Jan 28, 2014·Virus Research·Alexander Plyusnin, Tarja Sironen
Feb 4, 2014·Virus Research·Kumiko Yoshimatsu, Jiro Arikawa
May 24, 2014·Trends in Microbiology·Shannon N BennettRichard Yanagihara
Jul 6, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jiaxin LingOlli Vapalahti
Aug 16, 2014·Viruses·Kumiko Yoshimatsu, Jiro Arikawa
Aug 21, 2014·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·M GoeijenbierC B Reusken
Jan 8, 2015·Frontiers in Microbiology·Jussi HepojokiTomas Strandin

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