Serological survey with PCR validation for canine visceral leishmaniasis in northern Palestine

The Journal of Parasitology
Abedelmajeed NasereddinZiad A Abdeen

Abstract

Leishmania infantum is the causative agent of human and canine visceral leishmaniasis (CVL) in the Mediterranean region. A seroprevalence study for CVL was conducted in northern Palestine. Domestic dogs (n = 148) were screened for antileishmanial antibodies by enzyme-linked immunosorbent assay (ELISA). Ten dogs (6.8%) were seropositive. Promastigotes were isolated from one seropositive dog and identified as L. infantum by excreted factor (EF) serotyping, isozyme electrophoresis, and polymerase chain reaction (PCR). In addition to the ELISA, the internal transcribed spacer 1 (ITS1)-, modified ITS1 (mITS1)-, and kinetoplast DNA (kDNA)-PCRs were used to validate this technique as a diagnostic tool for CVL using blood; each assay was performed on 60 blood samples. kDNA-PCR (13/60 positives, 21.7%) was the most sensitive of the assays examined followed by mITS1-PCR (9/60, 15.0%), ELISA (5/60, 8.3%), and ITS1-PCR (3/60, 5%). However, ITS1-PCR and mITS1-PCR were also capable of identifying the parasite species and indicated they belong to L. infantum. In view of its higher sensitivity, kDNA-PCR is recommended for the routine diagnosis of CVL.

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Citations

Apr 15, 2011·Parasites & Vectors·Dmitriy A KovalenkoGad Baneth
Dec 25, 2009·Annals of Tropical Medicine and Parasitology·K Ait-OudhiaF Pratlong
Nov 7, 2008·Veterinary Parasitology·Abedelmajeed NasereddinZiad Abdeen
Jul 8, 2008·Trends in Parasitology·Guadalupe MiróGad Baneth
Dec 8, 2006·The Veterinary Journal·Y M GomesL C Alves
Jun 22, 2010·Tropical Medicine & International Health : TM & IH·Kifaya AzmiZiad Abdeen
Jul 29, 2020·The American Journal of Tropical Medicine and Hygiene·Ahmed Al-JawabrehAmer Al-Jawabreh

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Methods Mentioned

BETA
biopsies
enzyme-linked immunosorbent assay
PCR
ELISA
biopsy
electrophoresis
PCRs

Software Mentioned

BCM
SPSS

Related Concepts