Seroprevalence of Antibody-Mediated, Complement-Dependent Opsonophagocytic Activity against Neisseria meningitidis Serogroup B in England

Clinical and Vaccine Immunology : CVI
Holly E HumphriesStephen Taylor

Abstract

The correlate of protection for the licensure of meningococcal vaccines is serum bactericidal activity. However, evidence indicates that a complex situation and other mechanisms, such as antibody-mediated, complement-dependent opsonophagocytosis (OP), may play a role in protection and should be investigated in order to understand immunity to this disease. In this study, a high-throughput flow cytometric opsonophagocytic assay (OPA) was optimized. The assay measures the presence of killed fluorescently labeled Neisseria meningitidis within human granulocytes (differentiated HL60 cells) by flow cytometry, using IgG-depleted pooled human plasma as an exogenous source of complement. This method was found to be reliable and correlated with the results of an opsonophagocytic killing assay. The OPA was used to measure OP activity in 1,878 serum samples from individuals ranging from 0 to 99 years of age against N. meningitidis strain NZ98/254 (B:4:P1.7-2,4). The levels of OP activity in individual serum samples varied greatly. OP activity showed an initial peak in the 6- to 12-month age group corresponding to a peak in disease incidence. The OP activity dropped in childhood until the late teenage years, although there was still a highe...Continue Reading

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Citations

Jan 8, 2017·Annual Review of Pharmacology and Toxicology·Michael R Yeaman, John P Hennessey
Jun 15, 2017·Frontiers in Cellular and Infection Microbiology·Haiyong GuoYinduo Ji
Dec 20, 2019·Therapeutic Advances in Vaccines and Immunotherapy·Gabriela Trzewikoswki de LimaElizabeth De Gaspari

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