Seroprevalence study of human brucellosis by conventional tests and indigenous indirect enzyme-linked immunosorbent assay.

TheScientificWorldJournal
Annapurna S AgasthyaPrabhudas Krishnamsetty

Abstract

Brucellosis is one of the most important reemerging zoonoses in many countries. Brucellosis is caused by Gram-negative coccobacillus belonging to genus Brucella. Human brucellosis often makes the diagnosis difficult. The symptoms and clinical signs most commonly reported are fever, fatigue, malaise, chills, sweats headaches, myalgia, arthralgia, and weight loss. Some cases have been presented with only joint pain, lower backache, and involuntary limb movement, burning feet, or ischemic heart attacks. The focus of this work was to develop a highly sensitive and specific indirect ELISA by using smooth lipopolysaccharide antigen of Brucella abortus 99 to detect anti-Brucella antibodies at Project Directorate on Animal Disease Monitoring and Surveillance. Serum samples collected from 652 individuals in whom fever was not the major symptom but the complaint was of joint pain, headache, lower backache, and so forth, were screened by Rose Bengal plate agglutination test (RBPT) and standard tube agglutination test (STAT). Subsequent testing of sera by indigenous indirect ELISA detected 20 samples positive (3.6% seroprevalence), and indirect ELISA was found to be more sensitive than RBPT and STAT. The seroprevalence in South Karnataka w...Continue Reading

References

May 1, 1989·Postgraduate Medicine·D M RevakJ R Lubbers
Oct 1, 1968·Journal of Bacteriology·R DiazJ B Wilson
Jun 22, 1996·Lancet·D T BaddeleyC M Edwards
Apr 1, 1997·Emerging Infectious Diseases·F X Meslin
Jun 3, 2005·The New England Journal of Medicine·Georgios PappasEpameinondas Tsianos
Jul 31, 2007·Journal of Medical Case Reports·Farzin KhorvashAlireza Emami Naeini
Sep 29, 2007·Indian Journal of Medical Microbiology·C Vaishnavi, S Kumar

❮ Previous
Next ❯

Citations

Apr 4, 2014·Journal of Laboratory Physicians·Smita Mangalgi, Annapurna Sajjan
Apr 26, 2014·Emerging Health Threats Journal·Ajay D PathakSukhadeo B Barbuddhe
Jul 16, 2019·Zoonoses and Public Health·Nasrin Shirmohammadi-KhorramZohreh Maryanaji
Dec 10, 2016·Indian Journal of Medical Microbiology·D P PatilR D Kulkarni

❮ Previous
Next ❯

Methods Mentioned

BETA
Enzyme-linked immunosorbant assay
ELISA

Software Mentioned

STAT

Related Concepts

Related Feeds

Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Related Papers

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
F De MassisA Giovannini
The Spine Journal : Official Journal of the North American Spine Society
Lior DayanNimrod Rozen
Journal of Emergency Nursing : JEN : Official Publication of the Emergency Department Nurses Association
Rozanna Alperovich Penney
© 2021 Meta ULC. All rights reserved