Seroreactivity against glycolytic enzymes in inflammatory bowel disease

Inflammatory Bowel Diseases
Nathalie VermeulenXavier Bossuyt

Abstract

Patients with inflammatory bowel disease (IBD) carry autoantibodies such as perinuclear antineutrophil cytoplasmic antibodies. The aim of the current study was to further characterize the immune reactivity in IBD. We used an immunoproteomic approach with extracts from granulocytes and serum from ulcerative colitis (UC) patients and controls to identify target antigens. By means of Western blot analysis, we screened 60 UC and 60 Crohn's disease (CD) patients, 60 diseased, and 60 healthy controls for the antibodies. We performed gene array experiments on RNA extracted from colonic mucosal biopsies from 42 IBD patients and six controls. We identified aldolase A, phosphoglycerate mutase, alpha-enolase, triose-phosphate isomerase, and malate dehydrogenase as target antigens in IBD. Seroreactivity to at least one of these five antigens was detected in 53.3% of UC patients, 38.3% of CD patients, and 8.3% of controls. Seroreactivity to at least two antigens was detected in 16.7% of UC patients, 11.7% of CD patients, and none of the controls. Gene array experiments showed a significant upregulation of aldolase A, phosphoglycerate mutase, alpha-enolase, and pyruvate kinase mRNA in biopsies from IBD patients, but not controls. UC and CD p...Continue Reading

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Citations

May 3, 2011·Gastroenterology·Louise E Glover, Sean P Colgan
Apr 14, 2012·Xenotransplantation·Christopher BurlakA Joseph Tector
Jan 1, 2011·Japanese Clinical Medicine·Yui HiuraShinichi Moriwaki
Oct 12, 2017·Nature Reviews. Gastroenterology & Hepatology·Ashwin N AnanthakrishnanClaudio Fiocchi
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Jan 14, 2012·Current Opinion in Nephrology and Hypertension·Sandra M S HerrmannFernando C Fervenza
Jul 9, 2021·Frontiers in Molecular Biosciences·Carolina Rodríguez-SaavedraSobeida Sánchez-Nieto

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