Serotonin Alters the Phase Equilibrium of a Ternary Mixture of Phospholipids and Cholesterol

Frontiers in Physiology
Oskar EngbergDaniel Huster

Abstract

Unsaturated and saturated phospholipids tend to laterally segregate, especially in the presence of cholesterol. Small molecules such as neurotransmitters, toxins, drugs etc. possibly modulate this lateral segregation. The small aromatic neurotransmitter serotonin (5-HT) has been found to bind to membranes. We studied the lipid structure and packing of a ternary membrane mixture consisting of palmitoyl-oleoyl-phosphatidylcholine, palmitoyl-sphingomyelin, and cholesterol at a molar ratio of 4/4/2 in the absence and in the presence of 5-HT, using a combination of solid-state 2H NMR, atomic force microscopy, and atomistic molecular dynamics (MD) simulations. Both NMR and MD report formation of a liquid ordered (L o ) and a liquid disordered (L d ) phase coexistence with small domains. Lipid exchange between the domains was fast such that single component 2H NMR spectra are detected over a wide temperature range. A drastic restructuring of the domains was induced when 5-HT is added to the membranes at a 9 mol% concentration relative to the lipids. 2H NMR spectra of all components of the mixture showed two prominent contributions indicative of molecules of the same kind residing both in the disordered and the ordered phase. Compa...Continue Reading

References

Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D F Sargent, R Schwyzer
Mar 21, 1983·Biochimica Et Biophysica Acta·J H Davis
Oct 21, 1998·Biochemistry·W M YauS H White
Jan 19, 1999·Annual Review of Cell and Developmental Biology·D A Brown, E London
Nov 22, 2000·Neuron·D BrunsR Jahn
Jan 10, 2002·Journal of the American Chemical Society·Scott E FellerAlexander D MacKerell
Dec 17, 2004·Nature·Brent R Stockwell
Feb 14, 2006·Biochimica Et Biophysica Acta·Paulo F F AlmeidaAnne Hinderliter
May 10, 2006·Biochemistry·Alena SiarheyevaClemens Glaubitz
Oct 20, 2006·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Salvatore ChiantiaPetra Schwille
Feb 7, 2007·Physical Review Letters·Teemu MurtolaIlpo Vattulainen
Oct 6, 2007·Biochimica Et Biophysica Acta·Beate GriepernauRainer A Böckmann
Oct 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sarah L VeatchKlaus Gawrisch
Aug 21, 2008·Journal of the American Chemical Society·Olivier SoubiasKlaus Gawrisch
Sep 6, 2008·Biochimica Et Biophysica Acta·Luc BrunsveldDaniel Huster
Oct 9, 2008·Journal of the American Chemical Society·Tim BartelsMichael F Brown
Oct 14, 2008·Biophysical Journal·Beate Griepernau, Rainer A Böckmann
Oct 13, 2009·Biochemistry·Smita P SoniStephen R Wassall
Oct 16, 2009·Angewandte Chemie·Alexander VogelDaniel Huster
May 26, 2010·The Journal of Physical Chemistry. B·Jeffery B KlaudaRichard W Pastor
Jun 10, 2010·Journal of the American Chemical Society·Hansjörg JerabekThomas Stockner
Dec 8, 2010·Biochimica Et Biophysica Acta·Avigdor Leftin, Michael F Brown
Jul 19, 2006·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Alekos TsamaloukasHeiko Heerklotz
Sep 20, 2011·Biochimica Et Biophysica Acta·Olivier Soubias, Klaus Gawrisch
Jul 31, 2012·Biochimica Et Biophysica Acta·Nicole WeizenmannHolger A Scheidt
Jan 15, 2013·Journal of the American Chemical Society·Günther H PetersPeter Westh
Dec 18, 2013·Biochimica Et Biophysica Acta·Daniel Huster
Dec 19, 2013·Journal of the American Chemical Society·Alexander J SodtEdward Lyman
Jan 3, 2014·Angewandte Chemie·Holger A ScheidtDaniel Huster
May 14, 2014·Biochimica Et Biophysica Acta·Saame Raza ShaikhStephen R Wassall
Feb 7, 2015·Essays in Biochemistry·Ole G Mouritsen, Luis A Bagatolli

❮ Previous
Next ❯

Citations

Dec 18, 2020·Frontiers in Cell and Developmental Biology·Anna BochicchioRainer A Böckmann
Jan 28, 2021·Chemistry : a European Journal·Simli DeySudipta Maiti

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
AFM
force measurements
H
force
confocal microscopy

Software Mentioned

JPK
Mathcad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.