Serotonin and neuroendocrine peptides influence DNA synthesis in rat and human small intestinal cells in vitro

Acta Physiologica Scandinavica
K Zachrisson, A Uribe

Abstract

Animal studies suggest a mediator role for neuroendocrine peptides and amines in regulating cell proliferation in the gastrointestinal epithelium. Our aim was to examine the effect of serotonin and selected gastrointestinal peptides on DNA synthesis in a rat and human small intestinal cell line in vitro. IEC-6 and FHs-74 cells were incubated with epidermal growth factor (EGF), insulin-like growth factor II, glucagon, substance P, neurokinin A, calcitonin gene-related peptide (GRP, CCGRP), neurotensin and serotonin. The cells were labelled with [methyl-3H] thymidine and processed for autoradiography. DNA synthesis was evaluated by the labelling index. Epidermal growth factor, insulin-like growth factor II, glucagon, and substance P increased the labelling index in a dose-related manner (P < 0.003). In contrast, a significant dose-dependent reduction of the labelling index was observed after administration of serotonin and neurokinin A (P < 0.0001). Neurotensin and CGRP did not affect the labelling index. EGF, insulin-like growth factor II, glucagon, substance P, serotonin and neurokinin A may be important physiological regulators of proliferation, of gastrointestinal cells.

References

Jan 1, 1992·Journal of Nutritional Science and Vitaminology·T NagatsuK Titani
Mar 1, 1991·Pancreas·G E Feurle, S Niestroj
Mar 11, 1992·Digestive Diseases and Sciences·B M EversJ C Thompson
Nov 1, 1991·Experimental Physiology·R A GoodladN A Wright
Mar 15, 1990·Biochemical Pharmacology·K Seuwen, J Pouysségur
Jan 1, 1989·International Archives of Allergy and Applied Immunology·O Söder, P M Hellström
Apr 1, 1989·European Journal of Clinical Investigation·A UribeC Johansson
Jan 1, 1989·Acta Oncologica·F SundlerR Håkanson
Mar 1, 1987·Proceedings of the Society for Experimental Biology and Medicine·C N Conteas, A P Majumdar
May 16, 1988·Biochemical and Biophysical Research Communications·N WatanabeS Tarui
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G M NemecekM A Moskowitz
Jan 1, 1974·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·P J Tutton
Feb 1, 1984·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·E F StangeH Ditschuneit
Jul 1, 1997·European Journal of Gastroenterology & Hepatology·A UribeE Theodorsson

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Citations

Oct 26, 2006·Journal of Molecular Histology·Luzmarina HernandesEliana Parisi Alvares
Apr 1, 2000·Bioorganic & Medicinal Chemistry Letters·K M EdvinssonV Shcherbukhin
Apr 17, 2007·Annals of Surgery·Douglas J TurnerEric D Strauch
Nov 6, 2018·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·John J TackettRobert A Cowles
Jan 13, 2006·The Journal of Toxicological Sciences·Kazuaki NiikuraNoboru Chida
Feb 7, 2021·Cell and Tissue Research·Hirofumi KuramotoJohn B Furness

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