PMID: 8584426Jan 1, 1996Paper

Serotonin and protein kinase C modulation of a rat brain inwardly rectifying K+ channel expressed in xenopus oocytes

Pflügers Archiv : European journal of physiology
L DiMagnoW Schreibmayer

Abstract

In Xenopus laevis oocytes injected with rat brain poly(A)+ RNA, perfusion with a high-K+ solution (96 mM KCl) generated an inward current (IHK) which was absent in water-injected oocytes. Part of IHK was blocked by low concentrations of Ba2+ (half-maximal inhibitory concentration, IC50: 4.2 +/- 0.5 microM). When serotonin (5-HT) was applied to these oocytes a transient inward oscillating Cl- current arising from activation of Ca2+ -dependent Cl- channels, ICl (Ca), was observed. When this response decayed, a 30% reduction of IHK could be detected. Electrophysiological characterization of the K+ channel down-modulated by 5-HT revealed that it is an inward rectifier. Anti-sense suppression experiments revealed that the 5-HT2C receptor mediates the down-modulatory effect of 5-HT. The nature of the modulatory pathway was investigated by application of phorbol esters and intracellular injection of protein kinase C (PKC) inhibitors, ethylenebis (oxonitrilo)tetraacetate (EGTA) and inositol 1,4,5-trisphosphate. The results demonstrate that PKC is responsible for the down-modulatory effect.

References

Aug 1, 1991·The Biochemical Journal·M P KavanaughR A North
Feb 1, 1991·Neuron·J H HogerN Davidson
Feb 11, 1991·Nucleic Acids Research·M Sawadogo, M W Van Dyke
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T TakahashiB Sakmann
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M Apkon, J M Nerbonne
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J M BarabanB E Alger
Mar 1, 1994·Pflügers Archiv : European journal of physiology·W SchreibmayerN Dascal
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·B M VelimirovicY Nakajima
Feb 1, 1995·Trends in Neurosciences·S J Peroutka
Jun 1, 1995·Current Opinion in Neurobiology·C A DoupnikH A Lester
Dec 1, 1994·Neurochemistry International·F Saudou, R Hen
Sep 1, 1964·The Journal of General Physiology·S HAGIWARA, K I NAKA

❮ Previous
Next ❯

Citations

Mar 28, 2002·Pharmacology & Therapeutics·J R RaymondM N Garnovskaya
Jan 16, 1998·Cellular Signalling·N Dascal
Apr 1, 1997·Current Opinion in Cell Biology·L Y Jan, Y N Jan
Sep 11, 2007·Nature Neuroscience·Marie-Louise LunnPaul A Slesinger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.