Serotonin catabolism and the formation and fate of 5-hydroxyindole thiazolidine carboxylic acid.

The Journal of Biological Chemistry
Leah N SquiresJ V Sweedler

Abstract

Serotonin (5-HT) functions as a neurotransmitter and neuromodulator in both the central and enteric nervous systems of mammals. The dynamic degradation of 5-HT metabolites in 5-HT-containing nervous system tissues is monitored by capillary electrophoresis with wavelength-resolved laser-induced native fluorescence detection in an effort to investigate known and novel 5-HT catabolic pathways. Tissue samples from wild type mice, genetically altered mice, Long Evans rats, and cultured differentiated rat pheochromocytoma PC-12 cells, are analyzed before and after incubation with excess 5-HT. From these experiments, several new compounds are detected. One metabolite, identified as 5-hydroxyindole thiazoladine carboxylic acid (5-HITCA), has been selected for further study. In 5-HT-incubated central and enteric nervous system tissue samples and differentiated PC-12 cells, 5-HITCA forms at levels equivalent to 5-hydroxyindole acetic acid, via a condensation reaction between L-cysteine and 5-hydroxyindole acetaldehyde. In the enteric nervous system, 5-HITCA is detected without the addition of 5-HT. The levels of L-cysteine and homocysteine in rat brain mitochondria are measured between 80 and 140 microm and 1.9 and 3.4 microm, respective...Continue Reading

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Citations

Jan 12, 2013·Analytical and Bioanalytical Chemistry·Christopher A DaileyJonathan V Sweedler
Jun 12, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Elena V RomanovaJonathan V Sweedler
Jul 20, 2010·The Journal of Experimental Biology·Leah N SquiresJonathan V Sweedler
Nov 5, 2008·Analytical and Bioanalytical Chemistry·Philipp Schulze, Detlev Belder
Aug 5, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·D KeszthelyiA A M Masclee
May 12, 2015·Journal of Immunology Research·Rodrigo ArreolaLenin Pavón
Dec 7, 2007·Journal of Chromatography. a·Theodore Lapainis, Jonathan V Sweedler
Sep 20, 2007·Journal of Neurochemistry·Leah N SquiresJonathan V Sweedler

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