Serotonin Exhibits Accelerated Bleomycin-Induced Pulmonary Fibrosis through TPH1 Knockout Mouse Experiments

Mediators of Inflammation
Jingyao ZhangChang Liu

Abstract

Pulmonary fibrosis is a chronic progressive fibrosis interstitial lung disease that is characterized by inflammatory infiltration and fibrotic changes. 5-Hydroxytryptamine (5-HT) is an important regulatory factor in inflammation, immunomodulation, and fibrosis. The aim of this study was to investigate the role of 5-HT in bleomycin- (BLM-) induced pulmonary fibrosis through wild-type C57BL/6 (WT) and TPH1 knockout (KO) mouse experiments. The mice were grouped as follows: WT control group, KO control group, WT BLM group, and KO BLM group. Mice were administrated bleomycin hydrochloride through intratracheal instillation to induce pulmonary fibrosis. Mice were sacrificed 0, 7, 14, and 21 days after modeling, and bronchoalveolar lavage fluid (BALF) and lung tissues were collected to determine the severity of fibrotic changes. The results showed that the weight loss of mice in the WT BLM group was more severe than that in the KO BLM group. H&E and Sirius Red staining revealed that 5-HT markedly aggravated histological damage and fibrotic changes in the lung. Significantly lower levels of hydroxyproline, Ashcroft fibrosis score, total BALF protein and cells, BALF tumor necrosis factor- (TNF-) α and interleukin- (IL-) 6, TNF-α and IL-...Continue Reading

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Citations

Mar 3, 2020·Frontiers in Medicine·Argyris TzouvelekisDemosthenes Bouros
Aug 28, 2020·Free Radical Research·SunilGowda Sunnaghatta NagarajaDevipriya Nagarajan
Oct 21, 2019·Pharmacology & Therapeutics·Michael Bader
Oct 24, 2020·International Journal of Molecular Sciences·Roberta FuscoDaniela Impellizzeri
Jan 1, 2021·International Journal of Molecular Sciences·Anna LöfdahlGunilla Westergren-Thorsson
Apr 10, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lu YangJiang Deng

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Methods Mentioned

BETA
bronchoalveolar
lavage
Fluorescence
electrophoresis
MDA

Software Mentioned

GraphPad
ImageJ
Leica Application Suite Advanced Fluorescence
GraphPad Prism

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