Serotonin modulates transmitter release at central Lymnaea synapses through a G-protein-coupled and cAMP-mediated pathway

The European Journal of Neuroscience
P K McCamphillN I Syed

Abstract

Neuromodulation is central to all nervous system function, although the precise mechanisms by which neurotransmitters affect synaptic efficacy between central neurons remain to be fully elucidated. In this study, we examined the neuromodulatory action of serotonin [5-hydroxytryptamine (5-HT)] at central synapses between identified neurons from the pond snail Lymnaea stagnalis. Using whole-cell voltage-clamp and sharp electrode recording, we show that 5-HT strongly depresses synaptic strength between cultured, cholinergic neuron visceral dorsal 4 (VD4 - presynaptic) and its serotonergic target left pedal dorsal 1 (LPeD1 - postsynaptic). This inhibition was accompanied by a reduction in synaptic depression, but had no effect on postsynaptic input resistance, indicating a presynaptic origin. In addition, serotonin inhibited the presynaptic calcium current (I(Ca)) on a similar time course as the change in synaptic transmission. Introduction of a non-condensable GDP analog, GDP-beta-S, through the presynaptic pipette inhibited the serotonin-mediated effect on I(Ca.) Similar results were obtained with a membrane-impermeable inactive cAMP analog, 8OH-cAMP. Furthermore, stimulation of the serotonergic postsynaptic cell also inhibited p...Continue Reading

References

Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O BrahaE R Kandel
Jul 3, 1973·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R Miledi
Dec 1, 1974·The Journal of Physiology·H M Gerschenfeld, D Paupardin-Tritsch
Nov 1, 1967·The Journal of Physiology·F A Dodge, R Rahamimoff
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M Klein, E R Kandel
Oct 6, 1994·Nature·R HeidelbergerG Matthews
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K S SugamoriH H Van Tol
Sep 12, 1996·European Journal of Pharmacology·C C GerhardtH Van Heerikhuizen
Feb 5, 2002·Annual Review of Physiology·Robert S Zucker, Wade G Regehr
Nov 30, 2002·The Journal of Biological Chemistry·J David SpaffordNaweed I Syed
Feb 16, 1965·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·B KATZ, R MILEDI
Mar 8, 2005·Nature Neuroscience·Johann H Bollmann, Bert Sakmann
Feb 14, 2006·Journal of Neurobiology·Jon W JackletDavid G Tieman
May 2, 2006·Progress in Neurobiology·Jonathan R Wolpaw, Jonathan S Carp
Oct 28, 2006·The European Journal of Neuroscience·Haruo MizutaniTomoyuki Takahashi
Feb 9, 2007·Journal of Neurochemistry·Daniel S Cowen
Mar 30, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jong-Ju LeeYoung-Wuk Cho

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Citations

Aug 10, 2010·Biomedical Microdevices·Dolores MartinezGeoff Mealing
Aug 9, 2013·Journal of Neurophysiology·Tyler W Dunn, Wayne S Sossin
May 29, 2018·Progress in Neuro-psychopharmacology & Biological Psychiatry·Cheng Wei LuSu Jane Wang
Oct 19, 2010·The Journal of Experimental Biology·Jae Il-HanKen Lukowiak

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