Serotonin neurons derived from rhesus monkey embryonic stem cells: similarities to CNS serotonin neurons

Experimental Neurology
Ugur SalliCynthia L Bethea

Abstract

We sought an in vitro primate model for serotonin neurons. Rhesus monkey embryonic stem (ES) cell colonies were isolated and differentiated into embryoid bodies (EBs), then transferred to serum-free medium with 1% insulin-transferrin-selenium for 7 days to induce neural precursor cell (NPC) formation. NPCs were cultured in medium with 1% N-2 neural supplement and human fibroblast growth factor 2 (FGF2, 10 ng/ml) for 7 days to stimulate cell proliferation. Lastly, NPCs were dispersed into single cells and cultured without FGF2 for another 7 days to obtain terminal differentiation. Terminal cells were characterized for neuronal and serotonergic markers. Over 95% of the NPCs were immunopositive for nestin and Musashi1. Terminally differentiated cells appeared in both small and large morphologies. Most (>95%) of the mature cells (both small and large) were immunopositive for neuron-specific nuclear protein (NeuN), synaptophysin, microtubule-associated protein (MAP2C), Tau-1, neurofilament 160 (NF-160), beta-tubulin (TujIII), tryptophan hydroxylase (TPH), serotonin, the serotonin reuptake transporter (SERT), estrogen receptor-beta (ERbeta), and progestin receptor (PR), but not estrogen receptor-alpha (ERalpha). Less than 2-3% of cel...Continue Reading

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Citations

Nov 15, 2007·Stem Cells and Development·Miguel BartheleryKent E Vrana
Apr 9, 2008·Stem Cells and Development·Ugur SalliKent E Vrana
Feb 27, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Su-Chun ZhangMatthew T Pankratz
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