PMID: 2125270Dec 27, 1990Paper

Serotonin organelles of rabbit platelets contain synaptophysin

European Journal of Biochemistry
M BählerM Da Prada

Abstract

Synaptophysin, an integral membrane protein of synaptic vesicles in nerve terminals and a class of small translucent vesicles in neuroendocrine cells, was detected in intact rabbit platelets by immunoblotting, immunofluorescence staining and immuno-electron microscopy. In a highly purified preparation of serotonin organelles isolated from rabbit platelets, synaptophysin was enriched approximately 10-15-fold over platelet homogenate. About 80% of total platelet synaptophysin was present in this purified fraction. The apparent molecular mass (approximately 38 kDa) and the extent of glycosylation of platelet-derived synaptophysin was more similar to the neuronal than to the neuroendocrine form of the protein. Immunofluorescence microscopy revealed that synaptophysin was compartmentalized in intact rabbit platelets and immuno-electron microscopy of subcellular fractions showed that it was localized exclusively to the membrane surface of serotonin organelles. No synaptophysin-like immunoreactivity was detected in platelets from other species such as human, guinea pig and rat. Another integral membrane protein of synaptic vesicles, p65, and a family of synaptic vesicle-associated phosphoproteins, the synapsins, were not detected in p...Continue Reading

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Citations

Nov 1, 1996·The Prostate·M StridsbergG Ronquist
May 1, 1992·Journal of Cellular Biochemistry·A ShalevM Linial
Sep 1, 1992·Comparative Biochemistry and Physiology. Comparative Physiology·M A PackhamR L Kinlough-Rathbone
Jul 14, 1999·Thrombosis Research·A McNicol, S J Israels
Nov 20, 1998·Brain Research Bulletin·X Zheng, J A Bobich
Dec 14, 1999·The American Journal of Pathology·D CassimanT Roskams
Sep 24, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Tania RoskamsLouis Libbrecht
Oct 25, 2003·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·Kelly L RogersLyn R Griffiths

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