Serotonin receptor oligomerization regulates cAMP-based signaling

Journal of Cell Science
Sonal PrasadAndre Zeug

Abstract

Protein-protein interaction is often investigated using quantitative molecular microscopy with Förster resonant energy transfer (FRET). Here, we combined 'linear unmixing FRET' (lux-FRET) with the simultaneous application of a FRET-based biosensor for cAMP to investigate the oligomerization between the 5-HT7 receptor (5-HT7R, also known as HTR7) and the 5-HT1A receptor (5-HT1AR, also known as HTR1A) and its importance for cAMP signaling. We found that the 5-HT7R not only stimulates cAMP production, but also forms hetero-oligomers with 5-HT1AR, which blocks the inhibitory effect of the latter. 5-HT7R signaling, however, is not affected by this hetero-oligomerization. By modeling the kinetics of intracellular cAMP level changes in relation to the 5-HT7R:5-HT1AR stoichiometry, we were able to decipher the complex signaling characteristics of endogenous serotonin receptors in cultured hippocampal neurons. Our findings indicate that serotonergic signaling is not only modulated by the concentration of an individual receptor but also by its specific interaction with other receptors in endogenous systems. We conclude that the regulated ratio of serotonin receptors in immature and mature neurons may be critically involved in both the on...Continue Reading

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S FerreK Fuxe
Jan 1, 1990·Pharmacology & Toxicology·E Richelson
May 20, 1983·European Journal of Pharmacology·D N Middlemiss, J R Fozard
Jan 1, 1993·Molecular Neurobiology·M ZoliK Fuxe
Aug 5, 2000·Biochemical Society Transactions·F H MarshallA Wise
Jun 30, 2001·Trends in Pharmacological Sciences·M J Marinissen, J S Gutkind
May 24, 2003·Neuropharmacology·M R GuscottP H Hutson
Oct 30, 2003·Journal of Neuroscience Methods·Galina DityatevaAlexander Dityatev
Mar 23, 2004·European Journal of Pharmacology·Peter B HedlundPascal Bonaventure
Mar 8, 2005·Trends in Pharmacological Sciences·Sébastien BulengerMichel Bouvier
Aug 27, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Elena KvachninaEvgeni G Ponimaskin
Oct 31, 2006·Biochimica Et Biophysica Acta·Graeme Milligan
Aug 21, 2007·Ernst Schering Foundation Symposium Proceedings·G MilliganJ F Lopez-Gimenez
Oct 9, 2007·Biophysical Journal·Jakub WlodarczykErwin Neher
Apr 3, 2008·Biochimica Et Biophysica Acta·Fritz KobeEvgeni Ponimaskin
Apr 4, 2008·Trends in Pharmacological Sciences·Vsevolod V Gurevich, Eugenia V Gurevich
Sep 19, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Karima AzdadSerge N Schiffmann
Oct 1, 2008·Drug Discovery Today·Rosemarie Panetta, Michael T Greenwood
Oct 15, 2008·Glycoconjugate Journal·Andrew WoehlerEvgeni G Ponimaskin
Dec 22, 2009·Current Molecular Pharmacology·Andrew Woehler, Evgeni G Ponimaskin
Jan 12, 2010·Trends in Pharmacological Sciences·Raphael Rozenfeld, Lakshmi A Devi
Mar 2, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Fritz KobeEvgeni Ponimaskin
Jan 1, 2011·Frontiers in Endocrinology·Nicholas D HollidayAlastair J H Brown
Dec 4, 2012·Biophysical Journal·André ZeugEvgeni G Ponimaskin
Oct 23, 2013·Methods in Cell Biology·Agnieszka A KaczorAntti Poso
Oct 23, 2013·Methods in Cell Biology·Sonal PrasadEvgeni Ponimaskin
Jan 3, 2014·The British Journal of Nutrition·Graeme MilliganBrian D Hudson
Oct 18, 2014·Frontiers in Behavioral Neuroscience·Daria GusevaEvgeni Ponimaskin
Mar 26, 2016·Progress in Neurobiology·Alexander WirthEvgeni Ponimaskin
Mar 9, 2017·Frontiers in Cellular Neuroscience·Dasiel O Borroto-EscuelaKjell Fuxe
Dec 19, 2017·Cell·Alexander S HauserM Madan Babu

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Citations

Jan 17, 2020·International Journal of Molecular Sciences·Marianna CrispinoCarla Perrone-Capano
Jul 21, 2020·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jimmy OlusakinPatricia Gaspar
Aug 14, 2020·International Journal of Molecular Sciences·Gunnar KleinauPatrick Scheerer
Dec 18, 2020·Frontiers in Aging Neuroscience·Ji Yeon ChungJuhyun Song

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