Serotonin regulates β-casein expression via 5-HT7 receptors in human mammary epithelial MCF-12A cells

Biological & Pharmaceutical Bulletin
Takeshi ChibaKenzo Kudo

Abstract

We previously reported that serotonin (5-hydroxytryptamine; 5-HT) suppresses β-casein expression, a differentiation marker in mammary epithelial cells, via inhibition of the signal transducer and activator of transcription 5 (STAT5) phosphorylation in the human mammary epithelial cell line, MCF-12A. In this study, we investigated the expression pattern of the different 5-HT receptor subtypes in MCF-12A cells, and identified the receptors involved in 5-HT-mediated suppression of β-casein protein expression. β-Casein mRNA expression was inhibited by 30 µM 5-HT in a time-dependent manner. Treatment with 30 µM 5-HT for 72 h decreased β-casein protein levels and STAT5 phosphorylation (pSTAT5). The cells expressed four 5-HT receptors subtypes (5-HTR1D, 2B, 3A, and 7) at the mRNA and protein level, and their expression was elevated by prolactin (PRL) treatment. Additionally, the mRNA levels of 5-HTR1D and 5-HTR7 were significantly higher than the other 5-HT receptors in the cells. Tryptophan hydroxylase 1 mRNA was detectable in the cells in the absence of PRL, and PRL treatment significantly increased its expression. β-Casein and pSTAT5/STAT5 levels in the cells co-treated with 5-HT and a selective 5-HTR1D inhibitor, BRL15572, were eq...Continue Reading

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Citations

Feb 13, 2017·Biochemical and Biophysical Research Communications·Takeshi ChibaKenzo Kudo
Mar 27, 2016·Biochemical and Biophysical Research Communications·Takeshi ChibaKenzo Kudo
Jun 5, 2018·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takeshi ChibaKenzo Kudo
Dec 8, 2020·Frontiers in Cell and Developmental Biology·Min TianShihai Zhang

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