Serotonin release estimated by transcortical dialysis in freely-moving rats.

Neuroscience
E Carboni, G Di Chiara

Abstract

The transcerebral dialysis method has been utilized for measuring extracellular brain concentrations of serotonin and 5-hydroxyindolacetic acid. Dialysis fibres were implanted transversally in the rat frontal cortex and perfused by Ringer. Serotonin and 5-hydroxyindolacetic acid were quantified by reverse phase high performance liquid chromatography with electrochemical detection. Experiments were performed in freely-moving rats 20-24 h after the implant of the fibre. Basal output of serotonin and 5-hydroxyindolacetic acid was 0.12 and 22.8 pmol in 20 min, respectively. The output of serotonin was calcium-dependent and tetrodotoxin-sensitive (1 micron in the Ringer) while was stimulated by veratridine (50 microns) and by high concentrations of K+ (60 and 100 mM). Serotonin output was increased in a concentration-dependent manner by chlorimipramine (1-10 microM) in the Ringer; this drug stimulated serotonin release also when administered s.c. (20 mg/kg) in a tetrodotoxin-sensitive manner. The irreversible monoamine-oxidase inhibitor pargyline (75 mg/kg, i.p.) strongly stimulated serotonin output while reduced 5-hydroxyindolacetic acid output. A proposed serotonin releaser, fenfluramine (25 mg/kg, s.c.), stimulated serotonin rele...Continue Reading

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Citations

Mar 1, 1994·Neuropharmacology·K E Heslop, G Curzon
Apr 1, 1994·Pharmacology, Biochemistry, and Behavior·M E Olds
Sep 1, 1990·Journal of Neuroscience Methods·G Di Chiara
Mar 1, 1990·Trends in Pharmacological Sciences·G Di Chiara
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Jun 25, 2002·European Journal of Pharmacology·Nobuaki EgashiraMichihiro Fujiwara
Jan 29, 2002·Pharmacology, Biochemistry, and Behavior·José L Bata-GarcíaJosé L Góngora-Alfaro
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Dec 15, 2000·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·M T AbellánF Artigas
Jul 25, 2000·Brain Research. Brain Research Protocols·F FaddaR Stancampiano

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