PMID: 9171845Apr 1, 1997Paper

Serotype of Nigerian rotavirus strains

Tropical Medicine & International Health : TM & IH
M I AdahH Werchau

Abstract

Three hundred and fourteen stool samples collected from children < 5 years between December 1993 and August 1995 were analysed by PAGE, ELISA, PCR and Dot-blot hybridization technique for electropherotype and serotype distribution of rotavirus infection among Nigerian paediatric patients. 14.3% of the children were positive for rotavirus antigen. Children aged 6-9 months were most often infected, accounting for 35.6% of all positive samples, 91.1% of rotavirus-positive samples could be serotyped. Serotypes G2, G4 and G8 were not detected. Serotype G3 predominated (62.5%) in southern Nigeria, while mixed infection specificity was more widespread (63.6%) in northern Nigeria. The presence of some untypeable samples may indicate serotypes which the serotype-specific primers and cDNA probes used could not detect. Electropherotypes of 26 (57.7%) of the positive samples were determined. Two and 3 migration patterns were observed among the short and long-pattern electropherotypes, respectively. Implications for vaccine development and utilization in the country are discussed.

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Citations

Jul 13, 2002·Journal of Medical Virology·A D SteeleN E Gomwalk
Nov 10, 2016·Revista Do Instituto De Medicina Tropical De São Paulo·Babatunde Olanrewaju MotayoAdedayo Omotayo Faneye
Apr 4, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D D CardosoC M Soares
Aug 22, 2009·The Lancet Infectious Diseases·Elisabeth Sanchez-PadillaFrancisco J Luquero

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