Serotype-specific serum IgG antibodies to lipopolysaccharides of Pseudomonas aeruginosa in cystic fibrosis: correlation to disease, subclass distribution, and experimental protective capacity

Pediatric Research
U B SchaadE Fuerer

Abstract

Various studies have demonstrated pronounced systemic IgG response to Pseudomonas aeruginosa (PA) infection in cystic fibrosis (CF). However, antibody response to serotype-specific lipopolysaccharides (LPS) has never been studied. ELISA for detection of IgG antibodies to LPS of nine PA-serotypes and to toxin A were performed with serum of 78 CF patients. Anti-LPS profiles of antibodies were confirmed by SDS-PAGE and immunoblotting techniques. The most frequent PA-serotypes found were immunotypes (IT) IT-1 and IT-2, and Habs-3 and Habs-4. Ten patients without PA colonization showed no detectable antibody titers. In patients with chronic PA colonization (n = 46), these antibody titers were significantly (p less than 0.005) higher than in patients with intermittent PA colonization (n = 22). Mean serum antibody titers to LPS of PA IT-1, IT-2, Habs-3, and Habs-4 correlated with duration of PA colonization and with disease severity. Subclass analysis of anti-LPS antibodies revealed elevated levels for all four IgG subclasses and for IgA1. The IgG antibodies to LPS of PA proved to be protective in a murine burn wound sepsis model. We conclude that anti-LPS antibodies to specific PA serotypes in serum may be a sensitive measure of seve...Continue Reading

Citations

Oct 1, 1995·Immunology and Cell Biology·A W CrippsJ Kyd
Dec 11, 2008·Journal of Gastroenterology and Hepatology·Antonio M Morselli-Labate, Raffaele Pezzilli
Feb 1, 1992·Journal of Paediatrics and Child Health·K Grimwood
Sep 1, 1993·The American Review of Respiratory Disease·A Buret, A W Cripps
Apr 26, 2005·Journal of Immunological Methods·N R ThirumalapuraJ R Malayer

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