Serotype specificity of recombinant fusion protein containing domain III and capsid protein of dengue virus 2

Antiviral Research
Alienys IzquierdoMaría G Guzmán

Abstract

Recombinant fusion protein containing domain III of the dengue envelope protein fused to capsid protein from dengue 2 virus was immunogenic and conferred protection in mice against lethal challenge in previously report. Here, the antigenic specificity of this recombinant protein using anti-dengue antibodies from mice and humans and the cross-reactive humoral and cellular response induced in immunized mice were evaluated. The homologous anti-dengue antibodies showed a higher reactivity to the recombinant protein compared to the wide cross-reactivity observed for viral antigen as determined by ELISA. The IgG anti-dengue and functional antibodies, induced by the recombinant proteins in mice, were highly serotype specific by ELISA, hemaglutination inhibition and plaque reduction neutralizing tests. Accordingly, the cellular immune response determined by the IFNγ and TNFα secretion, was serotype specific. The specificity of serotype associated to this recombinant protein in addition to its high antigenicity, immunogenicity and protecting capacity suggest its advantage as a possible functional and safe vaccine candidate against dengue in a future tetravalent formulation.

References

Jan 1, 1995·Archives of Virology·E Simantini, K Banerjee
Mar 15, 2002·The Lancet Infectious Diseases·María G Guzmán, Gustavo Kourí
Jun 17, 2003·Nature Medicine·Juthathip MongkolsapayaGavin Screaton
Feb 18, 2005·The Journal of Infectious Diseases·Tadeusz J KochelKevin L Russell
Aug 31, 2006·Current Opinion in Infectious Diseases·Sharone Green, Alan Rothman
Sep 16, 2006·Journal of Virology·Karin StiasnyFranz X Heinz
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ana P GoncalvezChing-Juh Lai
Jul 25, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Ichiro Kurane
Sep 28, 2007·Clinical and Vaccine Immunology : CVI·Menaka D HapugodaN Khanna
May 15, 2008·Viral Immunology·John T RoehrigAlan D T Barrett
Jul 16, 2008·Antiviral Research·Lauren E Yauch, Sujan Shresta
Sep 18, 2008·Virus Research·Alienys IzquierdoMaría G Guzmán
Sep 16, 2009·Annals of the New York Academy of Sciences·Alan L Rothman
Jan 30, 2010·Expert Review of Vaccines·Maria G GuzmanGerardo Guillén
Apr 14, 2010·Expert Opinion on Therapeutic Patents·Sathyamangalam SwaminathanNavin Khanna
Apr 28, 2010·Immunology and Cell Biology·Heather FribergAnuja Mathew
Nov 17, 2010·Nature Reviews. Microbiology·Maria G GuzmanRosanna W Peeling

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Citations

Aug 19, 2016·Nature Reviews. Disease Primers·Maria G GuzmanScott B Halstead
Jan 25, 2019·Expert Review of Vaccines·Laura LazoLázaro Gil

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