SERPINB12 is a novel member of the human ov-serpin family that is widely expressed and inhibits trypsin-like serine proteinases

The Journal of Biological Chemistry
Yuko S AskewG A Silverman

Abstract

Members of the human serpin family regulate a diverse array of serine and cysteine proteinases associated with essential biological processes such as fibrinolysis, coagulation, inflammation, cell mobility, cellular differentiation, and apoptosis. Most serpins are secreted and attain physiologic concentrations in the blood and extracellular fluids. However, a subset of the serpin superfamily, the ov-serpins, also resides intracellularly. Using high throughput genomic sequence, we identified a novel member of the human ov-serpin gene family, SERPINB12. The gene mapped to the ov-serpin cluster at 18q21 and resided between SERPINB5 (maspin) and SERPINB13 (headpin). The presence of SERPINB12 in silico was confirmed by cDNA cloning. Expression studies showed that SERPINB12 was expressed in many tissues, including brain, bone marrow, lymph node, heart, lung, liver, pancreas, testis, ovary, and intestines. Based on the presence of Arg and Ser at the reactive center of the RSL, SERPINB12 appeared to be an inhibitor of trypsin-like serine proteinases. This hypothesis was confirmed because recombinant SERPINB12 inhibited human trypsin and plasmin but not thrombin, coagulation factor Xa, or urokinase-type plasminogen activator. The second-...Continue Reading

References

Apr 20, 1967·Biochemical and Biophysical Research Communications·I Schechter, A Berger
Jan 1, 1983·Annual Review of Biochemistry·J Travis, G S Salvesen
Sep 1, 1980·The Biochemical Journal·M O Longas, T H Finlay
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·S S SchneiderG A Silverman
Jan 4, 1993·FEBS Letters·E Remold-O'Donnell
Nov 12, 1998·Trends in Biochemical Sciences·F JeanmouginT J Gibson
Feb 20, 1999·The Journal of Biological Chemistry·S A GrigoryevC L Woodcock
Feb 1, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S CataltepeM P Upton
Aug 24, 2000·International Journal of Cancer. Journal International Du Cancer·Y UemuraG A Silverman
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Apr 28, 2001·The Journal of Biological Chemistry·A ZhouJ A Huntington
Jun 5, 2001·The Journal of Biological Chemistry·S De FalcoG Fassina

❮ Previous
Next ❯

Citations

Jan 11, 2005·Journal of Molecular Evolution·Anita J HorvathPaul B Coughlin
Dec 31, 2003·Methods : a Companion to Methods in Enzymology·Arumugam JayakumarGary L Clayman
Dec 17, 2008·Journal of Perinatology : Official Journal of the California Perinatal Association·D J Askew, G A Silverman
May 26, 2010·The Journal of Biological Chemistry·Gary A SilvermanPhillip I Bird
Jun 2, 2006·Genome Biology·Ruby H P LawJames C Whisstock
Nov 1, 2013·Human Genomics·Claire HeitVasilis Vasiliou
Jul 13, 2011·The Protein Journal·Fernando P P FonsecaFlavio Henrique-Silva
Oct 27, 2015·Biochemistry·Jason Z NiehausCliff J Luke
Mar 13, 2014·Animal Reproduction Science·D M HeringS Kaminski
Nov 7, 2008·Cognitive Neuropsychology·Aneta SzymaszekElzbieta Szelag
May 8, 2016·Psychological Reports·Irina Trofimova, Julie Christiansen
Jul 30, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jason Z NiehausCliff J Luke
May 3, 2002·The Journal of Biological Chemistry·Jerry E ChipukDavid Danielpour
Jul 22, 2008·Journal of Leukocyte Biology·Michael Bots, Jan Paul Medema
Apr 18, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ryuji YasumatsuSule Cataltepe
Aug 12, 2016·Turkish Journal of Medical Sciences·Hüseyin ÇelikÇetin Levent Peşkircioğlu
Jun 15, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gwenhael JégotBrice Korkmaz
Sep 20, 2006·Cancer Research·Xiaohua LiShijie Sheng
Dec 31, 2002·Archives of Biochemistry and Biophysics·Arumugam JayakumarGary L Clayman

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis