SERS-Based Lateral Flow Strip Biosensor for Simultaneous Detection of Listeria monocytogenes and Salmonella enterica Serotype Enteritidis

Journal of Agricultural and Food Chemistry
Hai-Bin LiuShuo Wang

Abstract

Rapid, sensitive, point-of-care detection of bacteria is extremely important in food safety. To address this requirement, we developed a new surface-enhanced Raman scattering (SERS)-based lateral flow (LF) strip biosensor combined with recombinase polymerase amplification (RPA) for simultaneous detection of Listeria monocytogenes and Salmonella enterica serotype Enteritidis. AuMBA@Ag core-shell nanoparticles were used in this SERS-LF. Highly sensitive quantitative detection is achieved by measuring the characteristic peak intensities of SERS tags. Under optimal conditions, the SERS intensities of MBA at 1077 cm-1 on test lines are used to measure S. Enteritidis (y = 1980.6x - 539.3, R2 = 0.9834) and L. monocytogenes (y = 1696.0x - 844, R2 = 0.9889), respectively. The limit of detection is 27 CFU/mL for S. Enteritidis and 19 CFU/mL for L. monocytogenes. Significantly, this SERS-LF has high specificity and applicability in the detection of L. monocytogenes and S. Enteritidis in food samples. Therefore, the SERS-LF is a feasible method for the rapid and quantitative detection of a broad range of bacterial pathogens in real food samples.

References

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Citations

Mar 29, 2020·The Analyst·Lei HuangJinhong Guo
Oct 24, 2019·Current Topics in Medicinal Chemistry·Nan ChengYunbo Luo
Jun 17, 2020·Critical Reviews in Food Science and Nutrition·Nuno M M PiresLuís F B A da Silva
Aug 11, 2020·Critical Reviews in Food Science and Nutrition·Yahui GuoAbdu Mahmud
Jan 23, 2021·Applied Microbiology and Biotechnology·Shuaishuai YanQing Liu
Dec 12, 2020·Advanced Science·Xia ZhouPinghua Sun
Jan 6, 2021·IET Nanobiotechnology·Sumana SannigrahiKrishnamurthy Suthindhiran
Nov 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ruiqi FanYiqiang Chen
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Yafang ShenYanbin Li
Feb 18, 2021·The Analyst·Chujun ZhengDaxiang Cui
Apr 27, 2021·Critical Reviews in Microbiology·Leonardo Lopes-LuzSamira Bührer-Sékula
May 4, 2021·Talanta·Elham SheikhzadehMohammed Zourob
May 28, 2021·Analytical and Bioanalytical Chemistry·Matheus Bernardes Torres FogaçaSamira Bührer-Sékula
May 30, 2021·Biosensors & Bioelectronics·Luyang WangLingxin Chen
Sep 11, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaojia LiuXing Ma
Feb 4, 2022·Analytical and Bioanalytical Chemistry·Sian Sloan-DennisonKaren Faulds

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