Serum 1alpha,25-dihydroxyvitamin D3 accumulates into the fracture callus during rat femoral fracture healing

Endocrinology
S JingushiY Iwamoto

Abstract

1,25-dihydroxyvitamin D3 (1,25(OH)2D3) is thought to be an important systemic factor in the fracture repair process, but the mechanism of action of 1,25(OH)2D3 has not been clearly defined. In this study, the role of 1,25(OH)2D3 in the fracture repair process was analyzed in a rat closed femoral fracture model. The plasma concentration of 1,25(OH)2D3 rapidly decreased on day 3 and continued to decrease to 10 days after fracture. We assessed whether this decrease was based on the accelerated degradation or retardation of the synthesis rate of 1,25(OH)2D3, from 25(OH)D3. After radiolabeled 3H-1,25(OH)2D3 or 3H-25(OH)D3 was injected i.v. into fractured or control (unfractured) rats, the concentrations of 25(OH)D3 and 1,25(OH)2D3 metabolites were measured by HPLC. The plasma concentrations of these radiolabeled metabolites in fractured group were similar to those in control rats early after operation. However, radioactivity in the femurs of fractured rats was higher than that of the control group. Furthermore, the radioactivity was concentrated in the callus of the fractured group analyzed by autoradiography. 1,25(OH)2D3 receptor gene expression was detected early after fracture and, additionally, both in the soft and hard callus o...Continue Reading

Associated Clinical Trials

References

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Citations

Dec 22, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Neil BhambMelodie Metzger
Apr 11, 2014·Trauma Monthly·Hossein EttehadArdeshir Shirangi
Dec 4, 2014·Journal of Clinical Periodontology·Alexander FüglGabriella Dvorak
Oct 1, 2015·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·V HegdeJ M Lane
Jan 29, 2016·Journal of Orthopaedic Trauma·Sheila SpragueGerard P Slobogean
May 15, 2019·Journal of Orthopaedic Trauma·Patrick F BerginMichael T Archdeacon
Dec 14, 2011·Clinical Oral Implants Research·Gabriella DvorakReinhard Gruber
Jan 26, 2021·Journal of Endocrinological Investigation·J VaculikJ J Stepan

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