PMID: 15383604Sep 24, 2004Paper

Serum amyloid A-activating factor-1 (SAF-1) transgenic mice are prone to develop a severe form of inflammation-induced arthritis

The Journal of Immunology : Official Journal of the American Association of Immunologists
Alpana RayB K Ray

Abstract

The transcription factor serum amyloid A-activating factor-1 (SAF-1) has been identified as a regulator of a number of cellular genes. To assess the pleiotropic role of SAF-1 in vivo, we generated SAF-1 transgenic mice, in which CMV immediate-early promoter was used to direct expression of the SAF-1 transgene in multiple organs. Our study shows that overexpression of SAF-1 predisposes animals to arthritis. Although SAF-1 transgenic mice do not spontaneously develop arthritis, they develop a severe form of arthritis when challenged with the Lyme disease agent Borrelia burgdorferi, which is known to promote arthritis development in both humans and mice. CMV-SAF-1 transgenic mice, upon B. burgdorferi infection, showed increased joint swelling and synovial inflammation compared with nontransgenic littermates. Immunohistochemical analysis of joint tissues collected 21 days after B. burgdorferi infection revealed colocalization of matrix metalloproteinase-1, a degradative enzyme that destroys type II collagen, a major architectural component of articular cartilage, and SAF-1 in both SAF-1 transgenic and nontransgenic mice. Further analysis by RNase protection assay and Western immunoblot demonstrated the presence of higher levels of ...Continue Reading

References

Nov 1, 1992·The American Journal of Tropical Medicine and Hygiene·S W BartholdA L Smith
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G C Kennedy, W J Rutter
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R D PalmiterR L Brinster
Aug 1, 1990·Molecular and Cellular Biology·E V SchmidtP Leder
Aug 31, 1989·The New England Journal of Medicine·A C Steere
Jan 1, 1985·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M G Ehrlich
Nov 1, 1987·Annals of Internal Medicine·A C SteereE Taylor
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·H Birkedal-HansenJ A Engler
Nov 1, 1995·Advances in Dental Research·W M Edgar, S M Higham
Jul 27, 1999·The Journal of Biological Chemistry·H Nagase, J F Woessner
Oct 3, 2000·Arthritis and Rheumatism·M B Goldring
Oct 6, 2000·Molecular Carcinogenesis·T D ColandreaK K Chada
Oct 2, 2002·Molecular Biotechnology·J Frederick Woessner
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charles R BrownChristie M Loiacono

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Citations

Aug 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alpana RayBimal K Ray
Sep 1, 2018·Molecular Medicine·George H Sack
Oct 8, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jackie K PaquetteJanis J Weis
Jan 24, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bimal K RayAlpana Ray
Jan 20, 2006·BMC Physiology·Marcia R SabanRicardo Saban

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