Serum amyloid A binds to fibrin(ogen), promoting fibrin amyloid formation

Scientific Reports
Martin J PageEtheresia Pretorius

Abstract

Complex associations exist between inflammation and thrombosis, with the inflammatory state tending to promote coagulation. Fibrinogen, an acute phase protein, has been shown to interact with the amyloidogenic ß-amyloid protein of Alzheimer's disease. However, little is known about the association between fibrinogen and serum amyloid A (SAA), a highly fibrillogenic protein that is one of the most dramatically changing acute phase reactants in the circulation. To study the role of SAA in coagulation and thrombosis, in vitro experiments were performed where purified human SAA, in concentrations resembling a modest acute phase response, was added to platelet-poor plasma (PPP) and whole blood (WB), as well as purified and fluorescently labelled fibrinogen. Results from thromboelastography (TEG) suggest that SAA causes atypical coagulation with a fibrin(ogen)-mediated increase in coagulation, but a decreased platelet/fibrin(ogen) interaction. In WB scanning electron microscopy analysis, SAA mediated red blood cell (RBC) agglutination, platelet activation and clumping, but not platelet spreading. Following clot formation in PPP, the presence of SAA increased amyloid formation of fibrin(ogen) as determined both with auto-fluorescence ...Continue Reading

References

Mar 1, 1985·Scandinavian Journal of Immunology·A HusebekkG Marhaug
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·N Eriksen, E P Benditt
Sep 1, 1993·Atherosclerosis·E MalleJ G Raynes
Feb 11, 1999·The New England Journal of Medicine·C Gabay, I Kushner
Oct 3, 1999·European Journal of Biochemistry·C M Uhlar, A S Whitehead
Feb 7, 2002·Blood·Simcha Urieli-ShovalYaacov Matzner
Feb 6, 2003·The Journal of Clinical Investigation·Weijun JinDaniel J Rader
Sep 23, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Denisa D Wagner, Peter C Burger
Jun 24, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Frederick C de Beer, Nancy R Webb
Feb 9, 2007·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Vance G NielsenWilliam L Holman
May 2, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Gerard MarxRaphael Gorodetsky
Nov 15, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Anisa JahangiriFrederick C de Beer
Jul 30, 2010·Journal of Lipid Research·Maria C de BeerFrederick C de Beer
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hyung Jin AhnSidney Strickland
Apr 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katri NiemiKari K Eklund
Sep 27, 2012·Biophysical Journal·Rustem I LitvinovJohn W Weisel
Nov 21, 2012·Cytokine·Myung-Hee KimFrederick C de Beer
Feb 20, 2013·The Analyst·Fiona T S ChanClemens F Kaminski
Jul 31, 2013·Current Opinion in Hematology·Prabhakara Nagareddy, Susan S Smyth
Dec 7, 2013·Journal of Lipid Research·Osamu MiyazakiTakafumi Kasumi
Mar 25, 2014·Clinical Rheumatology·Antonio VitaleLuca Cantarini
May 14, 2014·The Journal of Experimental Medicine·Hyung Jin AhnSidney Strickland
Jun 4, 2014·Cardiovascular Research·Marco van der StoepMiranda Van Eck
Sep 24, 2014·Methods in Molecular Biology·Jacky G GoetzJulien Vermot
Nov 12, 2014·Annual Review of Pathology·Gunilla T WestermarkPer Westermark
Apr 8, 2015·Acta Crystallographica. Section D, Biological Crystallography·Annette E LangkildeBente Vestergaard
Aug 1, 2015·Cardiovascular Diabetology·Prashilla Soma, Etheresia Pretorius
Aug 8, 2015·European Heart Journal·Stephen ZewingerWinfried März
Apr 3, 2016·Cardiovascular Diabetology·Prashilla SomaEtheresia Pretorius
May 22, 2016·Thrombosis Research·Daria Zamolodchikov, Sidney Strickland
Aug 27, 2016·Scientific Reports·Janette Bester, Etheresia Pretorius
Oct 25, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Etheresia PretoriusJanette Bester
May 23, 2017·Methods in Cell Biology·Claudia S LópezJoe W Gray

❮ Previous
Next ❯

Citations

May 29, 2019·Current Opinion in Lipidology·Preetha Shridas, Lisa R Tannock
May 20, 2020·Platelets·Maurizio TomaiuoloTimothy J Stalker
Jun 25, 2020·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Arnaud LionetMarc Hazzan
Jun 27, 2020·Diabetes/metabolism Research and Reviews·Carlos-Alberto Gonçalves, Patrícia Sesterheim
Aug 23, 2020·Life·Maria S RubelAleksandr A Rubel
Jun 6, 2019·Cardiovascular Diabetology·Shehan N RanderiaEtheresia Pretorius
Feb 29, 2020·Research and Practice in Thrombosis and Haemostasis·José A FernándezJohn H Griffin
Sep 12, 2019·Frontiers in Aging Neuroscience·Büin AdamsEtheresia Pretorius
Jan 16, 2021·Current Atherosclerosis Reports·Nancy R Webb
Mar 20, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Angelo ZinelluArduino A Mangoni
Jul 16, 2021·Arteriosclerosis, Thrombosis, and Vascular Biology·Ghadir AlkarithiRobert A S Ariëns
Jan 11, 2022·Acta Biomaterialia·Spencer R LynchNathan E Hudson

❮ Previous
Next ❯

Methods Mentioned

BETA
hormone replacement treatment
size-exclusion chromatography
light-electron microscopy
rheology
confocal microscopy
correlative microscopy
Light Electron Microscopy

Software Mentioned

Zeiss ZEN
ZEN
GraphPad Prism
ImageJ

Related Concepts

Related Feeds

Blood Coagulation Signaling Pathways

Coagulation is the process by which a blood clot is formed. This process includes both the formation of a platelet plug as well as a cascade of clotting factors resulting in the formation of fibrin strands. Find the latest research on coagulation signaling pathways here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.

Related Papers

Nihon rinsho. Japanese journal of clinical medicine
T Yamada
Nihon rinsho. Japanese journal of clinical medicine
Toshiyuki Yamada
Nihon rinsho. Japanese journal of clinical medicine
T Yamada
Rinsho byori. The Japanese journal of clinical pathology
T Yamada
Clinical Chemistry and Laboratory Medicine : CCLM
M Lammers
© 2022 Meta ULC. All rights reserved