Serum anti-EIF3A autoantibody as a potential diagnostic marker for hepatocellular carcinoma

Scientific Reports
Chang-Kyu HeoEun-Wie Cho

Abstract

Tumor-associated autoantibodies are promising diagnostic biomarkers for early detection of tumors. We have screened a novel tumor-associated autoantibody in hepatocellular carcinoma (HCC) model mice. Its target antigen was identified as eukaryotic translation initiation factor 3 subunit A (EIF3A) by proteomic analysis, and the elevated expression of EIF3A in HCC tissues of tumor model mice as well as human patients was shown. Also, its existence in tumor-derived exosomes was revealed, which seem to be the cause of tumor-associated autoantibody production. To use serum anti-EIF3A autoantibody as biomarker, ELISA detecting anti-EIF3A autoantibody in human serum was performed using autoantibody-specific epitope. For the sensitive detection of serum autoantibodies its specific conformational epitopes were screened from the random cyclic peptide library, and a streptavidin antigen displaying anti-EIF3A autoantibody-specific epitope, XC90p2(-CPVRSGFPC-), was used as capture antigen. It distinguished patients with HCC (n = 102) from healthy controls (n = 0285) with a sensitivity of 79.4% and specificity of 83.5% (AUC = 0.87). Also, by simultaneously detecting with other HCC biomarkers, including alpha-fetoprotein, HCC diagnostic sensi...Continue Reading

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Citations

Jul 4, 2020·Journal of Hematology & Oncology·Shu ZhangQiang Gao
Aug 23, 2020·Journal of Gastrointestinal Cancer·Dincer Ozgor, Emrah Otan
Nov 16, 2020·Genomics·Shi-Long JiangWen-Xu Hong
Jan 12, 2020·Seminars in Immunology·Makoto KobayashiSamir M Hanash
Dec 24, 2020·International Journal of Molecular Sciences·Chang-Kyu HeoEun-Wie Cho
Jul 3, 2021·International Journal of Molecular Sciences·Mengtao XingJian-Ying Zhang
Feb 6, 2020·Journal of Proteome Research·William E Fondrie, William S Noble

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Methods Mentioned

BETA
transgenic
Immunoprecipitation
FACS
ELISA
Size-exclusion chromatography
enzyme-linked immunosorbent assay
transfection
phage display
peptide display

Software Mentioned

Prism
GENT
Image J
GraphPad
EarlyCDT

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