Serum antibodies to heat shock protein 70 in sensorineural hearing loss

Archives of Otolaryngology--head & Neck Surgery
D B BlochK J Bloch

Abstract

To identify the 68-kd target of antibody in serum samples from patients with idiopathic, progressive, bilateral sensorineural hearing loss. To purify target protein from renal extracts using gel filtration, ion-exchange chromatography, and polyacrylamide gel electrophoresis and to transfer to nitrocellulose membranes. The purified protein was digested with trypsin, and peptide fragments were separated by high-pressure liquid chromatography. One fraction obtained by high-performance liquid chromatography contained a peptide of 2776 molecular weight. The sequence of a stretch of 22 amino acids within this peptide was identical to that of amino acids 424 through 445 of heat shock protein 70 (HSP70). On Western blotting, monoclonal antibody directed against HSP70 (but capable of recognizing both constitutive HSP70 [HSC70] and stress-inducible HSP70) reacted with the purified 68-kd protein. We compared the reactivity of serum samples from six patients with idiopathic, progressive, bilateral sensorineural hearing loss, as well as monoclonal antibody to HSC70, and monoclonal antibody to HSP70 with renal extract. The pattern obtained suggested that patient antibodies are preferentially directed at HSP70. The target of antibody in serum...Continue Reading

Citations

Dec 16, 1998·Lancet·R W Baloh
Aug 19, 2014·The Journal of Clinical Investigation·Steven D Rauch
Apr 16, 2004·The Journal of Clinical Investigation·C Arturo SolaresVincent K Tuohy
Apr 1, 2006·Cell Stress & Chaperones·Tangchun Wu, Robert M Tanguay
Sep 16, 2003·Cancer Treatment Reviews·Barbara A Jereczek-FossaRoberto Orecchia
Oct 13, 2006·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Marina SavastanoGino Marioni
Sep 9, 2008·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Roberto BovoAlessandro Martini
Dec 9, 2003·Journal of Immunological Methods·Leonardo MunariCristina Bonorino
Jan 1, 1999·Hearing Research·P B BillingsJ P Harris
May 31, 2011·Autoimmunity Reviews·A GrecoM De Vincentiis
Dec 15, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Bin ZhouTai June Yoo
Mar 26, 2009·The Laryngoscope·Pavan K KommareddiThomas E Carey
Sep 28, 2006·The Laryngoscope·Frank G OndreyJanine Smith
May 13, 1998·Neurologic Clinics·R W Baloh
Mar 1, 1997·The Laryngoscope·J Dhingra, M Mahalingam
Mar 6, 2015·Current Rheumatology Reports·Rodolfo Perez-Alamino, Hernán Maldonado-Ficco
Nov 5, 2011·PloS One·Irene GazquezJose A Lopez-Escamez
May 18, 2000·Otolaryngologic Clinics of North America·M J Ruckenstein
Jun 9, 1998·Annals of the New York Academy of Sciences·S D Rauch
Apr 1, 2006·The Annals of Otology, Rhinology, and Laryngology·Marina SavastanoGino Marioni
Sep 25, 2001·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·M U RahmanH K Choi
Sep 13, 2000·The Laryngoscope·S D RauchK J Bloch
Jul 3, 2013·Audiology & Neuro-otology·Akihiro J Matsuoka, Jeffrey P Harris
Dec 21, 2000·Clinical Otolaryngology and Allied Sciences·M R BoulasselM Gersdorff
Oct 22, 2014·World Journal of Methodology·David R LoboRafael Ramírez-Camacho
Sep 21, 2004·Current Opinion in Otolaryngology & Head and Neck Surgery·Michael J Ruckenstein
Jan 18, 2006·Current Opinion in Neurology·Charlotte Agrup, Linda M Luxon
Aug 23, 2006·Acta Oto-laryngologica·R BovoA Martini
May 16, 2007·Acta Oto-laryngologica·R Ramírez-CamachoJ A Vargas
Feb 15, 2001·Current Opinion in Neurology·A F RyanJ P Harris
Oct 3, 2007·The Journal of Laryngology and Otology·D LoboR Ramírez-Camacho
Jun 28, 2002·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·M Jennifer Derebery
Nov 22, 2011·The Annals of Otology, Rhinology, and Laryngology·Jeremy HornibrookJohn Gourley
Mar 29, 2001·Acta Oto-laryngologica·M R BoulasselM Gersdorff
Sep 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Moo-Jin BaekVincent K Tuohy
Oct 15, 2019·Pediatric Annals·Rotem Semo OzMelissa S Tesher
May 3, 2001·Current Opinion in Rheumatology·M U RahmanH K Choi
Oct 2, 2003·The Laryngoscope·Kristen YeomT E Carey
May 18, 1999·American Journal of Otolaryngology·M J Ruckenstein
Jun 14, 2021·Otolaryngologic Clinics of North America·Andrea Vambutas, Daniella V Davia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.

Related Papers

Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery
G R Holt
Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery
N T Shepard, S A Telian
© 2021 Meta ULC. All rights reserved