PMID: 6407959Apr 1, 1983Paper

Serum apolipoprotein AI synthesis in rat hepatocytes and its secretion as proform

Hoppe-Seyler's Zeitschrift für physiologische Chemie
W StoffelK Knyrim

Abstract

Rat hepatocytes in monolayer or suspension culture synthesize serum lipoprotein AI. It is secreted into the serum-free culture medium. Synthesis and secretion processes were studied in the presence of radiolabelled amino acids. The synthesis product of the hepatocytes and the secretion product from the medium were isolated by immunoprecipitation with a mono-specific rabbit antiserum against rat apolipoprotein AI. The intracellular and secreted products were homogeneous and identical in polyacrylamide gel electrophoresis but had reduced electrophoretic mobility as compared to native apolipoprotein AI. They were submitted to automated Edman degradation. They were present in their proform, the N-terminus of which is extended by a hexapeptide. In the presence of rat serum the proform is proteolytically transformed into the mature form of apolipoprotein AI.

References

Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Mar 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W StoffelR Deutzmann

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Citations

Sep 21, 1989·Biochimica Et Biophysica Acta·J L DixonD Banerjee
Nov 1, 1985·The Journal of Cell Biology·J M CaronM W Kirschner
Feb 1, 1985·Biological Chemistry Hoppe-Seyler·W StoffelM Burk
Feb 1, 1985·Biological Chemistry Hoppe-Seyler·W Stoffel, B Niedel
Jul 1, 1991·Biological Chemistry Hoppe-Seyler·W Stoffel, E Binczek
Nov 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W StoffelC Bode

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