Serum biomarkers identification by iTRAQ and verification by MRM: S100A8/S100A9 levels predict tumor-stroma involvement and prognosis in Glioblastoma

Scientific Reports
Anjali AroraKumaravel Somasundaram

Abstract

Despite advances in biology and treatment modalities, the prognosis of glioblastoma (GBM) remains poor. Serum reflects disease macroenvironment and thus provides a less invasive means to diagnose and monitor a diseased condition. By employing 4-plex iTRAQ methodology, we identified 40 proteins with differential abundance in GBM sera. The high abundance of serum S100A8/S100A9 was verified by multiple reaction monitoring (MRM). ELISA and MRM-based quantitation showed a significant positive correlation. Further, an integrated investigation using stromal, tumor purity and cell type scores demonstrated an enrichment of myeloid cell lineage in the GBM tumor microenvironment. Transcript levels of S100A8/S100A9 were found to be independent poor prognostic indicators in GBM. Medium levels of pre-operative and three-month post-operative follow-up serum S100A8 levels predicted poor prognosis in GBM patients who lived beyond median survival. In vitro experiments showed that recombinant S100A8/S100A9 proteins promoted integrin signalling dependent glioma cell migration and invasion up to a threshold level of concentrations. Thus, we have discovered GBM serum marker by iTRAQ and verified by MRM. We also demonstrate interplay between tumor mi...Continue Reading

References

Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·E C Holland
Mar 20, 2003·Microscopy Research and Technique·Wolfgang NackenClaus Kerkhoff
May 20, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Dirk FoellJohannes Roth
Jul 22, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Alexander HermaniDoris Mayer
Nov 14, 2007·Brain : a Journal of Neurology·C S JungM Sitzer
Feb 19, 2008·Clinical Chemistry·Vladimir PetrikMarios C Papadopoulos
Sep 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pratima SinhaGeetha Srikrishna
Nov 6, 2008·Acta Neuropathologica·Jörg BalssAndreas von Deimling
Dec 25, 2008·Journal of Proteome Research·Sheng PanTeresa A Brentnall
Feb 4, 2009·Journal of Proteome Research·Hye-Jung KimCheolju Lee
Oct 20, 2009·European Journal of Pharmacology·Saeid GhavamiClaus Kerkhoff
Apr 20, 2010·Cancer Cell·Houtan NoushmehrUNKNOWN Cancer Genome Atlas Research Network
Sep 14, 2011·Journal of Innate Immunity·Geetha Srikrishna
Feb 7, 2012·Immunology·Fei ZhaoFirouzeh Korangy
Mar 23, 2012·Nature Reviews. Immunology·Dmitry I GabrilovichVincenzo Bronte
May 1, 2012·Nature Biotechnology·Scott L CarterGad Getz
Oct 12, 2013·Nature Communications·Kosuke YoshiharaRoel G W Verhaak
May 3, 2014·Seminars in Oncology·Wael Al-ZoughbiGerald Hoefler
Oct 10, 2014·Proteome Science·Ionela Daniela PopescuCristiana Pistol Tanase
Apr 26, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kenta NarumiKazunori Aoki
May 6, 2015·International Journal of Mass Spectrometry·Lucía Geis-AsteggianteCatherine Fenselau
Nov 4, 2015·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob
Feb 10, 2016·Trends in Immunology·Vinit KumarDmitry I Gabrilovich
Feb 6, 2017·Molecular Cancer Research : MCR·Eva Schulze HeulingPhilipp Euskirchen
Mar 17, 2017·World Neurosurgery·Alice L HungMichael Lim
Nov 17, 2017·Genome Biology·Dvir AranAtul J Butte

❮ Previous
Next ❯

Citations

Feb 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laura KriegKristin Schubert
Dec 19, 2019·Frontiers in Oncology·Adil MalikJyotsna Batra
Jun 25, 2020·Expert Review of Proteomics·Khushman TaunkSrikanth Rapole
Mar 9, 2021·BMJ Neurology Open·Liyen Katrina KanMastura Monif
Jun 22, 2021·Frontiers in Oncology·Hamza AliMyron G Best
Jul 3, 2021·Cancers·James H ParkNitin S Baliga
Oct 5, 2021·Expert Review of Proteomics·Niraj BabuAditi Chatterjee

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE22866

Methods Mentioned

BETA
ELISA
trans-well

Software Mentioned

Proteome Discoverer (
SPSS
ESTIMATE

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Martin K HunnIan F Hermans
Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology
Peddagangannagari SreekanthreddyKumaravel Somasundaram
Cancer Biomarkers : Section a of Disease Markers
Jian ChenXiongwei Wang
© 2022 Meta ULC. All rights reserved