Serum Carbonic Anhydrase 1 is a Biomarker for Diagnosis of HumanSchistosoma mansoni Infection

The American Journal of Tropical Medicine and Hygiene
Manal I KardoushMomar Ndao

Abstract

AbstractSchistosoma mansoniis a major public health threat in many parts of the world. The current diagnostic tests for schistosomiasis are suboptimal, particularly early in infection, when the parasite burden is low and with reinfection after treatment. We sought to identify novel biomarkers of active infection by studying serum proteins in a mouse model of schistosomiasis followed by confirmation in chronically infected patients. Acute (6 weeks) and chronic (12 weeks) sera fromS. mansoni-infected C57Bl/6 mice as well as sera from chronically infected patients were assessed using two proteomic platforms: surface-enhanced, laser desorption and ionization, time-of-flight mass spectrometry and Velos Orbitrap mass spectrometry. Several candidate biomarkers were further evaluated by Western blot and/or enzyme-linked immunosorbent assay (ELISA). Among the most promising was carbonic anhydrase 1 (CA1), a host protein found primarily in red blood cells and enterocytes that proved to be a negative biomarker for schistosomiasis in both mouse and human samples. Reduced serum CA-1 levels were confirmed by both Western blot (murine and human: bothP< 0.001) and ELISA (human:P< 0.01). Western blots of serial mouse sera revealed a progressive...Continue Reading

References

Sep 1, 1977·The American Journal of Tropical Medicine and Hygiene·J E Byram, F von Lichtenberg
Dec 19, 1992·Lancet·M E JonesC L Leen
Apr 1, 1990·Gut·A R MohamedM I Yasawy
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C P KonialisP H Butterworth
Oct 16, 1995·The Medical Journal of Australia·C L Golledge
Feb 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·L G VisserP C Stuiver
Mar 1, 1994·Journal of Leukocyte Biology·A R CampbellE R Swenson
Mar 17, 1997·The Medical Journal of Australia·D B HipgraveB A Biggs
Jan 9, 1998·The Journal of Laboratory and Clinical Medicine·C E RobinsonA Keshavarzian
Sep 30, 1998·Digestive Diseases and Sciences·R FontiY Sambuy
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·T W Lane, F M Morel
Sep 15, 2000·Infectious Disease Clinics of North America·I BicaM J Stadecker
Jan 2, 2001·Transactions of the Royal Society of Tropical Medicine and Hygiene·C J WhittyP L Chiodini
Apr 9, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·T G MoreelsP A Pelckmans
Nov 7, 2001·The Journal of Biological Chemistry·B C TrippJ G Ferry
Jul 3, 2002·Nature Reviews. Immunology·Edward J Pearce, Andrew S MacDonald
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Jan 1, 2004·Trends in Parasitology·Michael J DoenhoffJoanne V Hamilton
Nov 27, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wu-Hsien KuoShu-Chen Chu
May 6, 2005·Nature·Todd W LaneFrançois M M Morel
Oct 27, 2005·Molecular & Cellular Proteomics : MCP·Jesper V OlsenMatthias Mann
May 23, 2006·International Immunopharmacology·Rashika El RidiHatem Tallima
Jun 27, 2008·Briefings in Functional Genomics & Proteomics·Mu WangDawn P G Brown
Sep 17, 2008·BMC Medical Genomics·Susann SchenkMatthias Mann
Mar 20, 2009·Current Genetics·Skander Elleuche, Stefanie Pöggeler
Sep 15, 2009·Biochimica Et Biophysica Acta·James G Ferry
Oct 17, 2009·Microbiology·Skander Elleuche, Stefanie Pöggeler
Dec 29, 2010·Journal of Proteome Research·Charlotte Welinder, Lars Ekblad
Oct 22, 2011·Asian Pacific Journal of Tropical Medicine·Salma Medani ElhagAhmed Babiker Mohamed
Mar 24, 2012·Clinical and Vaccine Immunology : CVI·Hans-Friedemann KinkelThomas Weitzel
Apr 27, 2013·Proteome Science·Sander R PiersmaConnie R Jiménez
Sep 11, 2013·Nature Reviews. Microbiology·Pierre-Edouard FournierDidier Raoult

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Citations

Jun 8, 2019·Expert Opinion on Therapeutic Patents·Sabina ZamanovaMarc A Ilies
Jun 22, 2019·Current Topics in Medicinal Chemistry·Josué de MoraesDaniel B Roquini
May 19, 2019·Science China. Life Sciences·Fanshuang ZhangYouhe Gao

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