Serum-dependent effects on adult and fetal tendon fibroblast migration and collagen expression

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Hallie E BrinkSteven B Nicoll

Abstract

Cell migration and extracellular matrix synthesis play an important role in the wound-healing response to injury. Several studies have described differences in migratory behavior and collagen biosynthetic activity in adult vs. fetal skin fibroblasts. The objective of this study was to examine the serum- and age-dependent effects on cell migration and collagen expression in tendon fibroblasts. Medial tendon fibroblasts were isolated from pregnant ewes and their fetuses, and cultured with and without serum for up to 7 days. Cell migration was determined by quantitative image analysis, and collagen expression was assessed by reverse transcription-polymerase chain reaction and immunohistochemical staining. In serum-containing medium, tendon fibroblasts migrated significantly faster than cells in serum-free medium. Additionally, fetal tendon fibroblasts migrated significantly faster than adult tendon fibroblasts under both culture conditions. The expression of types I and III collagen mRNA was significantly up-regulated in tendon cell populations in serum-free medium compared with those in serum-containing medium. Quantitative assessment of collagen staining indicated that fetal tenocytes produced more type I collagen than adult ten...Continue Reading

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Citations

Dec 30, 2014·Advanced Drug Delivery Reviews·Diana GasparDimitrios Zeugolis
Aug 18, 2009·Journal of Tissue Engineering and Regenerative Medicine·Hallie E BrinkSteven B Nicoll
Jan 1, 2014·Forensic Science International : Synergy·Marianne Cathrine RohdeJytte Banner
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Aug 22, 2018·Connective Tissue Research·Jack G GrahamPedro K Beredjiklian
Jul 24, 2008·Clinical Orthopaedics and Related Research·Simone S Stalling, Steven B Nicoll

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