Serum-dependent phosphorylation of human MAP4 at Ser696 in cultured mammalian cells

Cell Structure and Function
V SrsenS Hisanaga

Abstract

In the previous paper (Ookata et al., (1997) Biochemistry, 36: 249-259), we identified two mitotic cdc2 kinase phosphorylation sites (Ser696 and Ser787) in the proline-rich region of human MAP4. One (Ser696) of them was also phosphorylated during interphase. A protein kinase responsible for interphase phosphorylation of Ser696 could necessarily be distinct from cdc2/cyclin B kinase. To get insights into a physiological role for Ser696 phosphorylation, we searched for a Ser696 kinase and for cellular conditions under which Ser696 is dephosphorylated. Because Ser696 conforms to the MAP kinase phosphorylation consensus motif (PXSP), MAP kinase was tested as a possible kinase phosphorylating Ser696. MAP kinase, in fact, did phosphorylate Ser696 in MTB3, the carboxy-terminal half of human MAP4 in vitro. Phosphorylation of Ser696 in HeLa cell extract was suppressed by a MAP kinase inhibitor, DBTM-0004. Also consistent with the notion that Ser696 is a MAP kinase site were the fact that serum-starvation induced dephosphorylation of Ser696 in HeLa cells, TIG-3 and MRC-5-30 human fibroblasts, while readdition of serum recovered Ser696 phosphorylation, albeit after a surprisingly long interval. Thus, phosphorylation of Ser696 of MAP4, mos...Continue Reading

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Citations

Mar 9, 2007·Molecular Biology of the Cell·Per HolmfeldtMartin Gullberg
Jul 9, 2009·Cellular and Molecular Life Sciences : CMLS·Per HolmfeldtMartin Gullberg
Jun 13, 2006·Cell Motility and the Cytoskeleton·Daniel R Webster, Jason M Bratcher
Nov 30, 2005·Biotechnology and Bioengineering·Philip J LeeLuke P Lee
Oct 18, 2005·Biochemical and Biophysical Research Communications·Yao ChenDeyong Tan
Oct 6, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Amrita RamkumarKassandra M Ori-McKenney
Sep 29, 2020·Frontiers in Physiology·Lingfei LiJiongyu Hu
Feb 13, 2021·Cellular and Molecular Life Sciences : CMLS·Mitali ShahVaishnavi Ananthanarayanan

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