Serum-derived three-circRNA signature as a diagnostic biomarker for hepatocellular carcinoma

Cancer Cell International
Xiang-Hong SunLing Fan

Abstract

Hepatocellular carcinoma (HCC) is a common tumor characterized by high morbidity and mortality rates. The importance of circRNA in cancer diagnosis has been established. The study aimed to identify differentially-expressed circRNAs (DECs) in human blood exosomes from patients with HCC and to investigate their diagnostic value. The circRNA expression profiles of HCC and normal human blood samples were downloaded and processed from the exoRBase database. At the cutoff criteria of a fold change (FC) > 2.0 and P < 0.05, DECs were screened utilizing the limma package in the R software. A receiver operator characteristic curve (ROC) was used to study its diagnostic value. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis was performed to confirm the three-circRNAs expression in the blood samples with HCC. Various bioinformatics tools were used to characterize the potential biological pathways induced by circRNAs. Compared with the normal samples, seven up-regulated and five down-regulated circRNAs were determined in the HCC samples. ROC analyses demonstrated that hsa_circ_0004001, hsa_circ_0004123, hsa_circ_0075792, and a combination of the three biomarkers exhibited higher sensitivity and specificity. T...Continue Reading

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Citations

Sep 17, 2020·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·X GuoC Wang
Feb 28, 2021·Acta Pharmacologica Sinica·Hang LiSai-Jun Fan
May 30, 2021·Pathology, Research and Practice·Bashdar Mahmud HussenSoudeh Ghafouri-Fard
Jun 15, 2021·Canadian Journal of Gastroenterology & Hepatology·Mengnan GuoJinghua Wu
Jul 13, 2021·Frontiers in Oncology·Haoying WangYongning Zhou
Aug 12, 2021·Molecular Cancer Therapeutics·Wangyang ZhengYunfu Cui
Oct 29, 2021·Cancer Medicine·Natthaphong NimitrungtaweeNipon Chattipakorn

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Methods Mentioned

BETA
imaging techniques
RNA-Seq

Software Mentioned

SPSS
limma
Circular RNA Interactome
FunRich
R

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