PMID: 9555097May 23, 1998Paper

Serum DNA polymerase beta as an indicator for fatal liver injury of rat induced by D-galactosamine hydrochloride and lipopolysaccharide

Biochimica Et Biophysica Acta
O KatoS Yoshida

Abstract

DNA polymerase beta (pol beta) is a nuclear enzyme that is tightly bound to chromatin. Release of the pol beta activity into serum, therefore, may indicate the occurrence of massive destruction of cell nuclei in organs or tissues. In the present study, we made a liver injury model rat by the intraperitoneal injection of D-galactosamine hydrochloride (GalN, 500 mg/kg) and lipopolysaccharide (LPS, 100 microg/kg). Serum from the GalN/LPS-treated rats showed a high level of pol beta activity up to 118 pmol/0.5 microl serum (4700 cpm) at 12 h after the treatment, while the control rat serum showed the back ground level (3.8 pmol/0. 5 microl, 150+/-70 cpm). The serum pol beta activity was sensitive to inhibition by 2',3'-dideoxyTTP and by an anti-rat pol beta antibody. Among 30 rats treated with GalN/LPS, 10 rats died within 120 h (dead group). Serum pol beta activity in the dead group was as high as 23.0+/-19.5 pmol/0.5 microl (925+/-778 cpm) at 10 h after the treatment, while in alive group (n=20), it was 3.7+/-3.2 pmol. Levels of the serum pol beta activity correlated well with the prognosis of GalN/LPS-treated rats based on an analysis of the receiver-operator characteristic curves.

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C GalanosW Reutter
Jan 1, 1991·Annual Review of Biochemistry·T S Wang
Feb 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y ShiratoriT Sugimoto
Jun 18, 1971·Biochemical and Biophysical Research Communications·S YoshidaK Yagi
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May 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey C WallerAndrew D Hanson

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Citations

Sep 5, 2001·The Journal of Physiology·E A RichterJ F Wojtaszewski
Mar 19, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Olga SánchezMaria Soley

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