Serum exosomal microRNAs as potent circulating biomarkers for melanoma

Melanoma Research
Li TengdaDeng Anmei

Abstract

Exosomes are small homogenous membrane vesicles that derive from the exocytosis process of cells and can contain DNA, microRNAs (miRNAs), and/or proteins. Characterization of the content profile of exosomes may reflect the state of the cells that release them, and this could be predictive of disease. In this study, to explore the potential biomarkers for melanoma, we isolated serous exosomes from 30 patients with melanoma and 30 healthy individuals using the ultracentrifugation method. Five miRNAs were subsequently detected in each sample by quantitative reverse transcription-PCR: miRNA-532-5p, miRNA-106b, miRNA-200c, miRNA-199a-5p, and miRNA-210. Only the levels of exo-miRNA-532-5p and exo-miRNA-106b differed between the two groups (Z=-4.17 and -4.57, respectively, P<0.0001). When these two miRNAs were evaluated individually and in combination in 95 melanoma patients and 95 healthy individuals serum samples, the area under the receiver operating characteristic curve values were 0.867, 0.820, and 0.936, respectively. Furthermore, in blinded tests of samples from 25 melanoma patients and 25 healthy individuals, this panel of miRNAs identified 23/25 patients with melanoma (92.0% sensitivity) and 22/25 healthy individuals (88.0% s...Continue Reading

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Citations

Nov 21, 2019·Expert Review of Molecular Diagnostics·Sara CarpiPaola Nieri
Feb 15, 2020·Cells·Celeste LorussoStefania Tommasi
Sep 1, 2020·Journal of Cancer Research and Clinical Oncology·I-Peng ChenRüdiger Greinert
Jul 28, 2020·International Journal of Molecular Sciences·Aneta ZebrowskaMonika Pietrowska
Jul 2, 2020·International Journal of Molecular Sciences·Maria Letizia MottiMaria Vincenza Carriero
Feb 4, 2021·Pharmaceuticals·Florian HuemerRichard Greil
Feb 13, 2020·Cancer Treatment Reviews·Raghavendra GowdaGavin P Robertson
Mar 21, 2021·Journal of Experimental & Clinical Cancer Research : CR·Wenkang LuanJinlong Wang
May 11, 2021·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri
Jun 5, 2021·Advanced Drug Delivery Reviews·Ana ŠpilakChrista Noehammer
Aug 7, 2019·Seminars in Cancer Biology·Joshua L Hood
Jan 5, 2022·Photodermatology, Photoimmunology & Photomedicine·Liang DingGyorgy Paragh

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Methods Mentioned

BETA
transmission electron microscopy
flow cytometry
enzyme-linked immunosorbent assay
biopsies

Software Mentioned

Graph Prism
FlowJo
GraphPad
SPSS Statistics
Flow Jo

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