PMID: 13715478Jul 1, 1961Paper

Serum fractionation and the effects of bovine serum fractions on human cells grown in a chemically defined medium

The Journal of Biophysical and Biochemical Cytology


Serum has been fractionated by curtain electrophoresis using carboxymethyl cellulose dissolved in sodium bicarbonate electrolyte. Various fractions were produced from bovine serum and added to replicate cultures of Chang's endoepithelial cells and HeLa cells grown in a chemically defined medium. The effects of each of the various fractions on the appearance of the cultures and on cell multiplication were studied. Three different fractions were obtained and two were subjected to further purification. One fraction associated with albumin promoted survival, attachment, and flattening as well as cell multiplication. A second fraction associated with the alpha globulins promoted survival and multiplication of some cells. A third fraction caused cells to aggregate and form free floating clumps. An adequate chemically defined medium for continuous growth of human cells was used throughout the study. The response of cells to alterations in their environment which simulated some of the effects produced by serum fractions is described.


Sep 1, 1960·Experimental Cell Research·R W PUMPER, L J ALFRED
Oct 1, 1959·Proceedings of the Society for Experimental Biology and Medicine·R S CHANGH LIEPENS
Nov 17, 1954·Annals of the New York Academy of Sciences·W R EARLEE L SCHILLING
Dec 1, 1959·The Journal of Biophysical and Biochemical Cytology·R HOLMES
Jun 1, 1960·Proceedings of the Society for Experimental Biology and Medicine·R E NEUMAN, A A TYTELL

❮ Previous
Next ❯


Jul 1, 1979·In Vitro·R HolmesN Mohamed
Jan 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·T T PuckC Jones
Sep 1, 1966·The Journal of Cell Biology·G M Healy, R C Parker
Jul 1, 1969·The Journal of Cell Biology·R HolmesG Mercer
Jul 1, 1977·Experimental Cell Research·H Beug, T Graf
Aug 7, 1965·Nature·R W PumperL T Molander
Dec 1, 1969·Experimental Cell Research·C WallisJ L Melnick

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.