Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors.

Cytotechnology
V JägerP Friedl

Abstract

A serum free medium was developed, that could be used for the large scale propagation of various cell lines in bioreactors. The medium is based on a 1:1 mixture of Iscove's Modified Dulbecco's Medium and Ham's Medium F12, supplemented with transferrin, insulin and a BSA/oleic acid complex. Several myelomas, hybridomas derived from different myelomas and spleen cells, and other lymphoid and non-lymphoid cell lines were cultivated at growth rates comparable to those observed using serum-supplemented media. There was furthermore no reduction in the formation of products such as monoclonal antibodies or recombinant human interleukin-2.

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Citations

Jun 20, 1994·Biotechnology and Bioengineering·T RyllR Wagner
May 1, 1991·Cytotechnology·K RadfordP F Greenfield
Jan 1, 1991·Cytotechnology·G Hewlett
Jan 1, 1991·Cytotechnology·H BüntemeyerJ Lehmann
Jan 1, 1991·Cytotechnology·O W Merten, J Litwin
Nov 13, 2008·Cytotechnology·Joanne KeenanMartin Clynes
Sep 30, 1994·Journal of Immunological Methods·A L CauseyJ E Bly
Feb 1, 1992·Journal of Biotechnology·T Sugiura, H B Maruyama
May 31, 1994·Journal of Biotechnology·F R NayveH Kataoka
Jul 6, 2013·Lab on a Chip·John P WikswoWilliam J Matloff
Dec 1, 1993·Biotechnology and Bioengineering·R PolziusR D Schmid
Jan 6, 2012·The Journal of Nutritional Biochemistry·Jienny LeeDeokhoon Park
Jan 6, 2009·International Journal of Oral and Maxillofacial Surgery·H SchliephakeN Szubtarsky
Jun 5, 2008·Journal of Biomedical Materials Research. Part a·H SchliephakeT Wülfing
Jul 21, 2009·Cell Proliferation·J LeeD Park

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