PMID: 3772113Nov 6, 1986Paper

Serum-free in vitro bioassay for the detection of tumor necrosis factor

Journal of Immunological Methods
S M Kramer, M E Carver

Abstract

A sensitive, reproducible in vitro bioassay is described for quantitating the cytolytic activity of tumor necrosis factor (TNF). The assay target cells, murine connective tissue L-M, are propagated and the assay performed under serum-free conditions. The quantitation of cytolytic activity is based on the ability of TNF to lyse L-M cells in the presence of actinomycin D, as measured by crystal violet dye uptake of residual viable cells. The assay is sensitive to 88 pg/ml TNF-alpha. The simplicity of the culture medium combined with high sensitivity and low variability make this a particularly well-suited bioassay for routine detection of TNF cytolytic activity.

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