PMID: 7539670Feb 1, 1995Paper

Serum IgG response to Burkholderia cepacia outer membrane antigens in cystic fibrosis: assessment of cross-reactivity with Pseudomonas aeruginosa

FEMS Immunology and Medical Microbiology
D E LacyM R Brown

Abstract

Burkholderia cepacia (Pseudomonas cepacia) is now recognised as an important pathogen in cystic fibrosis patients, and several reports have suggested that sputum-culture-proven colonisation occurs despite the presence of specific antibody. In an attempt to establish the use of antibody studies as diagnostic and prognostic indicators of B. cepacia infection, we have examined the IgG response to B. cepacia outer membrane proteins and lipopolysaccharide in patients also colonised with P. aeruginosa. The B. cepacia strains were grown in a modified iron-depleted chemically defined medium and outer membrane components examined by SDS-PAGE and immunoblotting. IgG antibodies were detected against B. cepacia outer membrane antigens, which were not diminished by extensive preadsorption with P. aeruginosa. The response to B. cepacia O-antigen could be readily removed by adsorption of serum either with B. cepacia whole cells or purified LPS, whereas we were unable to adsorb anti-outer membrane protein antibodies using B. cepacia whole cells. The inability to adsorb anti-outer membrane protein antibodies using B. cepacia whole cells maybe due to non-exposed surface epitopes. Several B. cepacia sputum-culture negative patients colonised with...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Feb 1, 1984·The Journal of Pediatrics·A IslesH Levison
Feb 1, 1984·Journal of Clinical Microbiology·A I McKevitt, D E Woods
Apr 1, 1993·Journal of Clinical Microbiology·G Y LarsenJ L Burns

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Citations

Dec 11, 2008·Journal of Gastroenterology and Hepatology·Antonio M Morselli-Labate, Raffaele Pezzilli
May 28, 2013·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·P Gonçalves VidigalJ Steinmann
Jun 20, 2008·Innate Immunity·Anthony De SoyzaAntonio Molinaro

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