Serum immunoreactive activin A levels in normal subjects and patients with various diseases

The Journal of Clinical Endocrinology and Metabolism
K HaradaS Saito

Abstract

We developed and validated a RIA for measuring serum activin A. The least detectable value of this assay was 0.1 micrograms/L, and the antibody used cross-reacted slightly with bovine inhibin (3.2%) and porcine activin AB (10.0%) but not with porcine activin B (< 0.5%). Serum activin A was extracted with acetonitrile and trifluoroacetic acid to get rid of the interaction with possible binding proteins in serum. As a result of this extraction procedure, the dose-response curve of serum extract was parallel to the standard curve and a single immunoreactive (ir-) peak was demonstrated on gel chromatographic analysis with constant recovery rates over 80%. Serum ir-activin A level in healthy adults was 1.27 +/- 0.03 micrograms/L (mean +/- SEM, n = 180); being 1.38 +/- 0.05 micrograms/L (n = 90) in male, and 1.16 +/- 0.05 micrograms/L (n = 90) in female subjects, with a tendency to increase with age. Serum ir-activin A level during pregnancy showed a marked increase with the advance of gestation; 1.65 +/- 0.41 micrograms/L (n = 7) in the early, 4.50 +/- 1.13 micrograms/L (n = 21) in the middle, and 16.32 +/- 2.25 micrograms/L (n = 26) in the late trimester, with a rapid decline after delivery. On the other hand, serum ir-activin A le...Continue Reading

Citations

Nov 1, 2001·Journal of Endocrinological Investigation·P S MorpurgoA Spada
Jan 30, 2003·Journal of Endocrinological Investigation·M CentanniF Petraglia
Sep 23, 2009·Biochemical and Biophysical Research Communications·Indzi KatikJun-Ping Liu
Aug 30, 2018·Physiological Reviews·Jonathan W Lowery, Vicki Rosen
Feb 7, 2009·Journal of Applied Physiology·Arne YndestadArne K Andreassen
Jun 27, 2019·Pflügers Archiv : European journal of physiology·Anders NordholmEwa Lewin

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