Serum-induced iron-acquisition systems and TonB contribute to virulence in Klebsiella pneumoniae causing primary pyogenic liver abscess

The Journal of Infectious Diseases
Pei-Fang HsiehJin-Town Wang

Abstract

Klebsiella pneumoniae has become the predominant pathogen causing primary pyogenic liver abscess (PLA). K. pneumoniae was stimulated by human serum, and gene expression was analyzed by microarray. Three putative iron acquisition systems, Yersinia high-pathogenicity island (HPI), iucABCDiutA, and iroA(iroNDCB), that increased in expression and predominated in PLA-associated K. pneumoniae strains were identified. By use of siderophore uptake assays, these 3 systems were confirmed to be siderophore-dependent iron acquisition systems. Only the irp2-iuc-iroA triple mutant showed decreased virulence in mice. Full-genome analysis of K. pneumoniae strain NTUH-K2044 identified 10 putative iron uptake systems. Seven of these 10 systems were TonB dependent, including Yersinia HPI, iucABCDiutA, and iroA. A tonB deletion mutant was demonstrated to have profound attenuation of virulence. Immunization with the tonB mutant resulted in seroconversion of extracellular polysaccharide antibodies and protective efficacy against subsequent exposure to the parental strain. Iron uptake systems were the genes in K. pneumoniae that were highly up-regulated in response to sera. Although there are multiple iron transporter systems in NTUH-K2044, a mutatio...Continue Reading

References

Feb 1, 1989·Infection and Immunity·X NassifP J Sansonetti
May 1, 1988·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·R Reissbrodt, W Rabsch
Oct 1, 1987·Medical and Veterinary Entomology·L RyanE A Ishikawa
Oct 1, 1988·Journal of Clinical Gastroenterology·C T ChiuY F Liaw
Apr 1, 1985·The Journal of Infectious Diseases·S J CryzR Germanier
Nov 1, 1993·FEMS Microbiology Reviews·K G Wooldridge, P H Williams
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·A T MaurelliA Fasano
Jun 24, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J H WangH H Lin
Jun 27, 1998·Molecular Microbiology·G S Moeck, J W Coulton
Oct 6, 2000·Annual Review of Microbiology·C Ratledge, L G Dover
Mar 19, 2002·Emerging Infectious Diseases·Wen-Chien KoVictor L Yu
Apr 16, 2002·Infection and Immunity·Imke Hansen-Wester, Michael Hensel
Mar 26, 2003·DNA Sequence : the Journal of DNA Sequencing and Mapping·Wen-Sheng WuChao-Fu Chang
Aug 23, 2003·Infection and Immunity·Alfredo G Torres, James B Kaper
Jul 26, 2005·Infection and Immunity·Alfredo G TorresJ Gerardo Garcia-Gallegos
Jul 29, 2005·International Journal of Medical Microbiology : IJMM·Heather LavenderSteven Clegg
Feb 3, 2006·The Journal of Infectious Diseases·Yi-Ping ChuangJin-Town Wang
Apr 19, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Wen-Liang YuYin-Ching Chuang
Aug 24, 2006·Infection and Immunity·Matthew S LawlorVirginia L Miller
Dec 19, 2006·The Journal of Infection·D R ChungUNKNOWN Korean Study Group for Liver Abscess
Jan 16, 2007·Infection and Immunity·Matthew S LawlorVirginia L Miller

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Citations

Jun 6, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Y KeynanP Lagacé-Wiens
Jun 21, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nai-Chen ChengChi-Tai Fang
May 18, 2011·Infection and Immunity·Michael A BachmanJeffrey N Weiser
Feb 1, 2011·Journal of Bacteriology·Christa H ChatfieldNicholas P Cianciotto
May 24, 2014·PloS One·Yi-Chyi LaiYing-Tsong Chen
Jun 19, 2012·Future Microbiology·Alyssa S Shon, Thomas A Russo
Nov 12, 2013·Microbes and Infection·Wei-Sheng W SunShao-Hung Wang
Dec 18, 2013·Future Microbiology·Zhenhong ChenDongsheng Zhou
Mar 13, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Wei LiHao Wu
Jan 13, 2012·Comparative Immunology, Microbiology and Infectious Diseases·Karen M HoldenMarc S Marenda
Oct 3, 2015·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Chi-Tai FangShih-Feng Tsai
Dec 3, 2014·Microbial Pathogenesis·Kuang-Ming ChenYi-Chyi Lai
Oct 24, 2014·Future Microbiology·Bei LiDongsheng Zhou
Sep 9, 2008·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Yi-Jung ChenPo-Ren Hsueh

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