PMID: 11324719Apr 28, 2001Paper

Serum induces a transcriptional activation of CYP1A1 gene in HepG2 independently of the AhR pathway

Life Sciences
N GuigalY Barra

Abstract

CYP1A1 is largely implicated in carcinogenesis. To date, it is known that this gene is induced by xenobiotics such as polycyclic aromatic hydrocarbons. In this study, we evaluated the effect of serum in the regulation of CYP1A1 gene expression. CYP1A1 mRNA level is induced 1) in HepG2 and HT29-D4 cells by 3-methylcholanthrene 2) only in HepG2 after treatment by serum. The CYP1A1 mRNA induction in HepG2 is the consequence at least in part of a transcriptional activation as was demonstrated by evaluation of the hnRNA level. HepG2 cells were transfected by a plasmid containing the 7.5 Kb of the CYP1A1 promoter and the CAT reporter gene. No CAT stimulation was observed after serum treatment. These results demonstrated that CYP1A1 is induced at a transcriptional level by a physiological compound contained in serum independently of the Ah receptor and the 7.5 Kb promoter region.

Citations

Sep 3, 2008·Analytical and Bioanalytical Chemistry·Christina U Köhler, Peter H Roos
Mar 12, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sara E BassGrace Chao Yeh
Nov 1, 2003·Archives of Biochemistry and Biophysics·Scott A MonkRobert H Rice
Dec 19, 2002·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Q FengT Osawa
Nov 7, 2007·International Journal of Cancer. Journal International Du Cancer·Henry P CiolinoGrace Chao Yeh
Aug 3, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J JanosekI Holoubek
Oct 30, 2004·International Journal of Toxicology·Aramandla RameshEric H Weyand
Jan 27, 2004·Molecular Pharmacology·Aviva Levine-FridmanCornelis J Elferink
Sep 19, 2002·Biochemical and Biophysical Research Communications·Quoc-Binh N'GuyenYves Barra

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