Serum induction of the fibroblast growth factor-binding protein (FGF-BP) is mediated through ERK and p38 MAP kinase activation and C/EBP-regulated transcription

Oncogene
V K HarrisA Tate Riegel

Abstract

The fibroblast growth factor-binding protein (FGF-BP) modulates FGF activity through binding and release from the extracellular matrix. Consequently, the expression of FGF-BP in certain tumor types is a rate-limiting regulator of FGF-mediated angiogenesis. FGF-BP is upregulated in squamous cell carcinoma by treatment with mitogens such as EGF or TPA. In this study, we investigated the regulation of FGF-BP gene expression by serum. Treatment of serum-starved ME-180 cells with fetal bovine serum (FBS) resulted in a rapid increase in steady-state levels of FGF-BP mRNA and in the rate of FGF-BP gene transcription. Serum induction of FGF-BP mRNA was not mediated through EGF receptor activation but was dependent on PKC, as well as ERK kinase (MEK) and p38 MAP kinase activation. Promoter analysis showed that C/EBP is the main promoter element required for the serum response. Unlike EGF-activation of FGF-BP, transcriptional induction by serum is not significantly regulated through the AP-1 or E-box sites in the promoter. These results illustrate differences between the mechanism of induction in response to serum and EGF.

References

Jan 1, 1991·Annual Review of Biochemistry·H R Herschman
Aug 30, 1996·The Journal of Biological Chemistry·E D Liaudet-Coopman, A Wellstein
Feb 1, 1996·In Vitro Cellular & Developmental Biology. Animal·T OkamotoJ D Sato
May 1, 1997·Biochemical Society Transactions·S YangA D Sharrocks
Nov 7, 1998·The Journal of Biological Chemistry·G J DarlingtonO A MacDougald
Nov 13, 1998·The Journal of Biological Chemistry·A M Diehl
Nov 21, 1998·The Journal of Biological Chemistry·J A EngelmanP E Scherer
Apr 6, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H Rochefort, E Liaudet-Coopman
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
Dec 2, 1999·Experimental Cell Research·E S Kandel, N Hay
Dec 2, 1999·Experimental Cell Research·J EnglishM H Cobb
Apr 17, 2001·Australian Veterinary Journal·P D KirklandK G Hart

❮ Previous
Next ❯

Citations

Jul 21, 2006·The Journal of Biological Chemistry·Hemant KhannaAnand Swaroop
Nov 19, 2004·Neoplasia : an International Journal for Oncology Research·Andreas KurtzAnton Wellstein
May 13, 2006·Biochemical and Biophysical Research Communications·Victorino R BrionesRobert J Lechleider
Dec 6, 2005·The International Journal of Biochemistry & Cell Biology·Shaker AbuharbeidAchim Aigner
Jul 13, 2005·Biochemical and Biophysical Research Communications·Tove BergMagnus Nord
Oct 7, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rolf ZiescheLutz-Henning Block
Jul 17, 2008·Biological Chemistry·Koushik Chakrabarty, Rolf Heumann
Feb 24, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Wenjie HuangLimin Xia
Dec 13, 2003·The Journal of Biological Chemistry·Pelin Kelicen, Niclas Tindberg
Jul 18, 2001·The Journal of Biological Chemistry·M CattaruzzaM Hecker

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.