PMID: 7015247Apr 1, 1981Paper

Serum isoamylases determination. Analytical and critical study of a new method using an inhibitor protein

Pathologie-biologie
M O BenoitR G Douris

Abstract

A new method for the serum isoamylase assay is studied. Its principle is the use of an inhibitor protein from wheat, mainly active on the salivary type isoamylases. Amylase remaining in the presence of the inhibitor (R) and total amylase (T) are measured and the R/T ratios calculated. The kinetic of the inhibition is studied with various samples of pancreatic juice and saliva, of which the isoenzymatic pattern has been evidenced by electrofocusing. The results show that 80% of the salivary type isoamylases (S) and 20% of those from pancreatic type (P) are inhibited in the operational conditions described. The inhibition is stopped, but not modified, by addition of amylase activity substrate. The analytic study of the kit showed convenient results. The chemical inhibition methodology is compared with a modified Davies' electrophoretic technic on cellulose acetate membrane. The results are shown on a diagram: R/T ratio on the ordinate and total amylasemia on the abscissa. In the middle of the diagram appears an area which corresponds to normal values obtained with 69 healthy subjects from 20 to 30 years old.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie
M T ParviainenH Jokela
Hepato-gastroenterology
E V TsianosJ M Braganza
© 2021 Meta ULC. All rights reserved