Oct 1, 1975

Serum lactate dehydrogenase activity ratios with different substrates

Clinical Biochemistry
D BarlowJ King

Abstract

1. The lactate dehydrogenase activity of 89 sera from patients suffering myocardial infarction and of 55 sera from patients with hepatocellular damage was assayed under optimal conditions using pyruvate, alpha-oxobutyrate, hydroxypyruvate and glyoxylate as substrates. Activity was also measured with lactate as substrate at different pH values. 2. The ratios of activities under these different assay conditions were calculated for both series of patients. Correct differentiations for single ratios ranged from virtually nil for hydroxypyruvate/alpha-oxobutyrate to is greater than 93 per cent for glyoxylate/hydroxypyruvate and glyoxylate/alpha-oxobutyrate. This was little improved by the use of multiple ratios involving up to seven separate assays. 3. The activity ratio of hydroxypyruvate to pyruvate which is consistently greater than unity was found to be inverted in a case of morphine poisoning.

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Mentioned in this Paper

Lactate
Morphine Measurement
Liver Carcinoma
Pyruvate Measurement
Myocardium
Myocardial Infarction
Poisoning Aspects
Liver Damage
Phosphoglycerate Dehydrogenase Activity
Lactic Acid Measurement

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