Serum-mediated Activation of Bone Marrow-derived Mesenchymal Stem Cells in Ischemic Stroke Patients: A Novel Preconditioning Method

Cell Transplantation
Gyeong Joon MoonOh Young Bang

Abstract

Stroke induces complex and dynamic, local and systemic changes including inflammatory reactions, immune responses, and repair and recovery processes. Mesenchymal stem cells (MSCs) have been shown to enhance neurological recovery after stroke. We hypothesized that serum factors play a critical role in the activation of bone marrow (BM) MSCs after stroke such as by increasing proliferation, paracrine effects, and rejuvenation. Human MSCs (hMSCs) were grown in fetal bovine serum (FBS), normal healthy control serum (NS), or stroke patient serum (SS). MSCs cultured in growth medium with 10% SS or NS exhibited higher proliferation indices than those cultured with FBS ( P < 0.01). FBS-, NS-, and SS-hMSCs showed differences in the expression of trophic factors; vascular endothelial growth factor, glial cell-derived neurotrophic factor, and fibroblast growth factor were densely expressed in samples cultured with SS ( P < 0.01). In addition, SS-MSCs revealed different cell cycle- or aging-associated messenger RNA expression in a later passage, and β-galactosidase staining showed the senescence of MSCs observed during culture expansion was lower in MSCs cultured with SS than those cultured with NS or FBS ( P < 0.01). Several proteins rela...Continue Reading

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Citations

Mar 28, 2019·Frontiers in Neurology·Oh Young Bang, Eun Hee Kim
Oct 16, 2020·Journal of Stroke·Shuai ZhangXiaofeng Jia
Nov 3, 2020·Frontiers in Cellular Neuroscience·Inês BarrosCatarina O Miranda
Jul 6, 2021·Stem Cells International·Yinghan GuoGao Chen
Jan 22, 2021·Neurology·Jong-Won ChungUNKNOWN STARTING-2 Collaborators

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Methods Mentioned

BETA
flow cytometry
Polymerase Chain Reaction
PCR
Antibody Array
chip
Fluorescent-activated cell sorting

Clinical Trials Mentioned

NCT01716481

Software Mentioned

Graph Pad Prism
Cell Quest
SPSS
Image
Graph Pad
FunRich
STAT

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