Serum metabolome and targeted bile acid profiling reveals potential novel biomarkers for drug-induced liver injury

Medicine
Zhenhua MaJunqi Niu

Abstract

This study aims to determine the non-invasive, reliable and sensitive biochemical parameters for the diagnosis of drug-induced liver injury (DILI).Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) and selected reaction monitoring (SRM) were used to profile the serum metabolome and quantify 15 targeted bile acid metabolites, respectively, in samples obtained from 38 DILI patients and 30 healthy controls.A comparison of the resulting serum metabolome profiles of the study participants revealed significant differences between DILI patients and healthy controls. Specifically, serum palmitic acid, taurochenodeoxycholic acid, glycocholic acid (GCA), and tauroursodeoxycholic acid (TUDCA) levels were significantly higher, and serum lysophosphatidylethanolamine levels were significantly lower in DILI patients vs healthy controls (P < .001). Furthermore, the SRM assay of bile acids revealed that the increase in GCA, taurocholic acid (TCA), TUDCA, glycochenodeoxycholic acid (GCDCA), glycochenodeoxycholic sulfate (GCDCS), and taurodeoxycholic acid (TDCA) corresponded to a higher degree of liver damage. These results also indicate that serum concentrations of chenodeoxycholic acid (CDCA), deoxycholic acid (...Continue Reading

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Citations

Jul 22, 2020·Antimicrobial Agents and Chemotherapy·Carlijn H C LitjensJan B Koenderink
Jun 2, 2020·Toxicology·Venkat R PannalaAnders Wallqvist
Jul 8, 2020·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Qiuju TianJidong Jia
Jun 5, 2021·Toxicological Sciences : an Official Journal of the Society of Toxicology·Thomas KraljDarren J Creek
Aug 12, 2021·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Zeinab FarhatErikka Loftfield
Nov 4, 2021·Expert Opinion on Drug Metabolism & Toxicology·Edmond AtallahGuruprasad P Aithal

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Methods Mentioned

BETA
biopsies
metabolomics profiling
PCA
biopsy

Software Mentioned

SIMCA
PLS
GraphPad Prism
Excel
DA
Metabolomics
SIEVE
SPSS

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